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MB Sample ID: SA019472
| Local Sample ID: | Fox-A |
| Subject ID: | SU000427 |
| Subject Type: | Animal |
| Subject Species: | Felis catus |
| Taxonomy ID: | 9685 |
| Species Group: | Mammal |
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Treatment:
| Treatment ID: | TR000441 |
| Treatment Summary: | The care of the 53 cats in the study followed the NIH Guide for the Care and Use of Laboratory Animals. The University of Missouri Animal Care and Use Committee approved the study design (ACUC protocols #6912 and 7891). In order to induce allergic asthma, on day 0, cats <1 year of age were administered 12 µg of Bermuda grass allergen (BGA) in 10 mg of alum and 100 ng Bordetella pertussis toxin (both subcutaneously). On day 14, intranasal BGA (75 µg of BGA in 200 µL of phosphate-buffered saline) was given; and on day 21, 12 µg of BGA in 10 mg alum was injected subcutaneously. On day 28, the formation of wheals on an intradermal test confirmed sensitization to BGA. For the next 2 weeks, intensive aerosol challenges were administered to all cats with BGA (500 µg of BGA in 4 mL of phosphate-buffered saline) over 10 min in awake and unrestrained cats in a sealed plastic chamber. A nebulizer (Acorn nebulizer, model 646, Devilbis Health Care, Somerset, PA) delivered an aerosol of the BGA solution at an air flow rate of 9.3 l /min. An air compressor (Easy Air 15, Precision Medical, Inc., Northampton, PA) supplied the compressed air flow at a pressure of 2.93 kg/cm2. At week 6, samples were collected 24 h after challenge with BGA aerosol (see below). |
| Treatment Compound: | Bermuda grass allergen (BGA) |
