Return to study ST000468 main page
MB Sample ID: SA023660
| Local Sample ID: | OG1RF_2 Mup |
| Subject ID: | SU000489 |
| Subject Type: | Bacterial cells |
| Subject Species: | Enterococcus faecalis |
| Taxonomy ID: | 1351 |
| Genotype Strain: | OG1RF |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR000503 |
| Treatment Summary: | Triplicate cultures of E. faecalis OG1RF will be grown in FMC-AUG to OD600 = 0.25. The cultures will be split into 3 equal volumes of 30mL which will be exposed to 100uL DMSO, 0.01mg/mL mupirocin, or 0.1mg/mL decoyinine for 15 minutes. At this point, 10mL sample will be removed for protein analysis. The remaining 20mL will be harvested by fast filtration for analysis.|Triplicate cultures of E. faecalis OG1RF will be grown in FMC-AUG to OD600 = 0.25. The cultures will be split into 3 equal volumes of 30mL which will be exposed to 100uL DMSO, 0.01mg/mL mupirocin, or 0.1mg/mL decoyinine for 15 minutes. At this point, 10mL sample will be removed for protein analysis. The remaining 20mL will be harvested by fast filtration for analysis.|Triplicate cultures of E. faecalis OG1RF will be grown in FMC-AUG to OD600 = 0.25. The cultures will be split into 3 equal volumes of 30mL which will be exposed to 100uL DMSO, 0.01mg/mL mupirocin, or 0.1mg/mL decoyinine for 15 minutes. At this point, 10mL sample will be removed for protein analysis. The remaining 20mL will be harvested by fast filtration for analysis. |
| Treatment Compound: | DMSO|Mupirocin|Decoyinine |
| Treatment Dose: | 100uL|0.01mg/mL|0.1mg/mL |
| Treatment Doseduration: | 15 min|15 min|15 min |
| Treatment Vehicle: | DMSO|DMSO|DMSO |
