Return to study ST001094 main page
MB Sample ID: SA074547
| Local Sample ID: | sample.11 |
| Subject ID: | SU001138 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR001152 |
| Treatment Summary: | For the global metabolomics with a focus on the lipids: Cells (passage <20) were maintained in CMRL 1066 media containing 10% FBS and 1% glutamate in a humidified atmosphere at 37˚ C, 5% CO2, and 95% air (Osgood et al. 2013). Once cells were grown to confluence, cells were serum deprived for 24 hr prior to treatment with the binary 1:1 LMW PAH mixture of 1-MeA and Flthn at a final dose of 40 μM. This mixture has been used previously in our lab (PMC5808746) to represent two LMW PAH’s common in secondhand smoke and environmental exposures. |
| Treatment Protocol Filename: | AKB_Untargeted-protocol.docx |
| Treatment Protocol Comments: | For AKB study 1 protocol, the collection, treatment, and sample prep are all in the same file. |
