Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA094558

Local Sample ID:	9404 +Gly 3
Subject ID:	SU001382
Subject Type:	Bacteria
Subject Species:	Salmonella enterica serovar Typhimurium
Taxonomy ID:	90371
Genotype Strain:	LT2
Gender:	Not applicable
Subject Comments:	The wild type as describe, also used a ridA null mutant (DM3480, ridA3::MudJ1734), DM9404

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Treatment:

Treatment ID:	TR001397
Treatment Summary:	Ten biologically independent wild-type and ridA mutant cultures were grown overnight in NB medium shaking at 37 °C and used to inoculate (1% inoculum) 250 mL non-baffled flasks holding 125 mL of medium. Each culture inoculated one each of the three media types (minimal medium, minimal medium with 1 mM L-isoleucine, and minimal medium with 1 mM glycine), for a total of 60 flasks. Flasks were randomly arranged in an Innova®44 incubator and cultures allowed to grow 16 h at 37 °C, shaking at 180 RPM. Cultures were chilled 5 min on ice and then harvested by centrifugation at 7,000 x G for 10 min at 4 °C. The supernatant was decanted, with 10 mL transferred to sterile 15 mL conical tubes and flash-frozen using liquid nitrogen for downstream analysis of external metabolites. Cell pellets were transferred to sterile 15 mL conical tubes after resuspension in 10 mL ddH2O, prior to a second pelleting at 7,000 x G for 10 min at 4 °C. The supernatant was decanted and pellets were flash-frozen using liquid nitrogen and stored at -80 °C.

Treatment ID:

TR001397

Treatment Summary:

Ten biologically independent wild-type and ridA mutant cultures were grown overnight in NB medium shaking at 37 °C and used to inoculate (1% inoculum) 250 mL non-baffled flasks holding 125 mL of medium. Each culture inoculated one each of the three media types (minimal medium, minimal medium with 1 mM L-isoleucine, and minimal medium with 1 mM glycine), for a total of 60 flasks. Flasks were randomly arranged in an Innova®44 incubator and cultures allowed to grow 16 h at 37 °C, shaking at 180 RPM. Cultures were chilled 5 min on ice and then harvested by centrifugation at 7,000 x G for 10 min at 4 °C. The supernatant was decanted, with 10 mL transferred to sterile 15 mL conical tubes and flash-frozen using liquid nitrogen for downstream analysis of external metabolites. Cell pellets were transferred to sterile 15 mL conical tubes after resuspension in 10 mL ddH2O, prior to a second pelleting at 7,000 x G for 10 min at 4 °C. The supernatant was decanted and pellets were flash-frozen using liquid nitrogen and stored at -80 °C.