Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA151730

Local Sample ID:	Inulin_Media_1
Subject ID:	SU001728
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Gender:	Female

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Treatment:

Treatment ID:	TR001741
Treatment Summary:	Fresh fecal samples (ca. 110 mg) from each mouse were weighed and dispensed in 3 mL of prepared medium separately in an anaerobic chamber. The samples were pestled to suspend the microorganisms and particles. The suspensions were subjected to low-speed centrifugation and the supernatants were then collected and centrifuged to pellet microbes. The microbial cells were then suspended in 4 mL of culture medium, and divided equally into two Hungate tubes. To each paired tube was amended with either 13C-Inulin or 13C-cellulose aseptically to achieve a final concentration of 4 g/L fibers. The sealed Hungate tubes were incubated in a water bath (37°C) for 24 h. After incubation, the samples were centrifuged (3,000 g, 5 min) to collect supernatant (culture medium). Each pellet was washed with 1 mL of fresh culture medium and centrifuged to collect the microbial cells. All procedures were performed under anaerobic conditions.

Treatment ID:

TR001741

Treatment Summary:

Fresh fecal samples (ca. 110 mg) from each mouse were weighed and dispensed in 3 mL of prepared medium separately in an anaerobic chamber. The samples were pestled to suspend the microorganisms and particles. The suspensions were subjected to low-speed centrifugation and the supernatants were then collected and centrifuged to pellet microbes. The microbial cells were then suspended in 4 mL of culture medium, and divided equally into two Hungate tubes. To each paired tube was amended with either 13C-Inulin or 13C-cellulose aseptically to achieve a final concentration of 4 g/L fibers. The sealed Hungate tubes were incubated in a water bath (37°C) for 24 h. After incubation, the samples were centrifuged (3,000 g, 5 min) to collect supernatant (culture medium). Each pellet was washed with 1 mL of fresh culture medium and centrifuged to collect the microbial cells. All procedures were performed under anaerobic conditions.