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MB Sample ID: SA151798
| Local Sample ID: | Mus_M406_LPS_Plasma |
| Subject ID: | SU001731 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus;Peromyscus leucopus |
| Taxonomy ID: | 10090;10041 |
| Gender: | Male and female |
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Treatment:
| Treatment ID: | TR001744 |
| Treatment Summary: | Animals were anesthetized with isoflurane and injected i.p. with a single dose of E. coli O111:B4 LPS at a concentration of 10 µg/gm body weight in a 50 µl volume as described above. The control group was anesthetized and then injected with the 0.9% saline alone. The experiment started at 0800 h with 10 min intervals between animals and with alteration of LPS and control injections. At 4.0 hr after their injection the animals were euthanized. After opening the chest, exsanguination was performed by cardiac puncture and blood was transferred to a heparin sulfate coated tubes (Becton-Dickinson Microtainer). Anticoagulated blood was centrifuged to pellet blood cells for 3 min at 4600 x g at 4° C. Plasma and blood pellet was kept separately at -80° C until further analysis. |
| Treatment Compound: | E. coli O111:B4 LPS |
| Treatment Route: | i.p. |
| Treatment Dose: | 10 µg/gm body weight |
| Treatment Dosevolume: | 50 µl volume |
| Treatment Vehicle: | sterile, endotoxin-free 0.9% saline |
| Animal Anesthesia: | isoflurane |
