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MB Sample ID: SA235123
| Local Sample ID: | COLO205_neg_05 |
| Subject ID: | SU002430 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Treatment:
| Treatment ID: | TR002442 |
| Treatment Summary: | SW480 cells and SW620 cells were cultured in DMEM medium supplemented with 10% FBS at 37 °C under a 5% CO2 atmosphere, HT-29 and COLO 205 cells were cultured in RPMI 1640 medium supplemented with 10% FBS at 37 °C under a 5% CO2 atmosphere. |
| Cell Media: | DMEM medium supplemented with 10% FBS is for SW480 cells and SW620 cells, while RPMI 1640 medium supplemented with 10% FBS is for HT-29 and COLO 205 cells. |
| Cell Envir Cond: | at 37 °C under a 5% CO2 atmosphere |
| Cell Harvesting: | After discarding the medium in each culture dish, the cells were quickly rinsed twice with cold isotonic saline (0.9% NaCl [w/v], 4 °C). Water (1.5 mL) was added to each dish, and then the dishes were stored in a freezer (−80 °C) for 20 min before extraction. Then, cells were collected by scraping with a cell scraper. |
| Cell Pct Confluence: | 70%-80% |
