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MB Sample ID: SA255042

Local Sample ID:Purslane_CP1_Adult_Leaf_Lipidic_Negative
Subject ID:SU002637
Subject Type:Plant
Subject Species:Portulaca oleracea
Taxonomy ID:46147

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Treatment ID:TR002649
Treatment Summary:The B1 accession of purslane (Portulaca oleracea L.) used in this study belongs to the Purslane Collection at Embrapa Agroenergia. Seeds underwent disinfection following the same procedure described in Belo Silva et al. (2022), which consisted of soaking in 2% sodium hypochlorite and Tween® 20 for five minutes, under slow agitation, and then washing with sterile water and drying on sterilized filter paper. After being seeded on a culture medium (MS 1/2 strength, Phytagel 0.2%, and pH 5.8) (Murashige and Skoog, 1962), it was kept for germination in a Growth chamber Conviron mod. Adaptis 1000TC (Controlled Environments Ltd, Winnipeg, Canada) at 150 μmol/m2/s of light and 30°C. After 13 days, seedlings were individually transferred to 200 ml plastic cups containing 100 g of sterilized substrate - clay soil, vermiculite, and a commercial substrate (Bioplant®), 2:1:1 (v:v:v) ratio – and transferred to another Conviron® growth chamber mod. PGW40 at 25±2°C, 500±20 μmol/m2/s of light, 65±5% air relative humidity, and photoperiod of 16/8 h (light/dark), and kept there until the end of the experiments. The plants were allowed to acclimatize for three days, and the salinity stress started three weeks after the end of the acclimatization period. The salinization experiment consisted of two salinity levels (0.0 and 2.0 g of NaCl / 100 g of the substrate), with 16 replicates (plants) in a completely randomized design, and the stress lasted 12 days. During the entire experiment, plants were at field capacity. To avoid the loss of Na+ or Cl-, no leakage of the saline solution was allowed to get out of the plastic cup, as described previously in Belo Silva et al. (2022). The water lost due to evapotranspiration was replaced with deionized water daily, and the electric conductivity at field capacity (ECfc) and water potential in the substrate solution was measured once - on the 8th day of stress - for all replicates.