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MB Sample ID: SA258836
| Local Sample ID: | Bin67_No Glutamine_1 |
| Subject ID: | SU002680 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Treatment:
| Treatment ID: | TR002692 |
| Treatment Summary: | BIN-67 cells were plated in RPMI supplemented with 6% dialyzed FBS (Wisent Bio, Cat# 080-950) at ~80% confluency the day before experiments. The next day, BIN-67 cells were cultured in the absence of glucose or glutamine for 24h. Cells were washed twice in cold saline solution (NaCl, 0.9 g/l) and metabolites were extracted with 1 ml 80% ice-cold methanol (GC/MS grade). |
