Summary of Study ST000313

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000252. The data can be accessed directly via it's Project DOI: 10.21228/M86S3T This work is supported by NIH grant, U2C- DK119886.

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Study IDST000313
Study TitleMuscle Clock knock out mice metabolic changes (iMSBmal1-Exp1)
Study TypeRegular
Study SummaryMy lab studies the function of the molecular clocks in skeletal muscle. We have an inducible genetic mouse model (C57Bl6 background) in which we knock out the core clock gene, Bmal1, only in adult skeletal muscle after treatment with tamoxifen. We have found that the mice maintain body mass but lose fat mass at 10 weeks after loss of Bmal1. We have done expression profiling on the skeletal muscles and gene expression changes (insulin signaling, CHO metabolism, fat metabolism) suggest significant changes in substrate metabolism. To analyze TCA, CHO metabolites we have collected gastrocnemius muscles from these mice following instructions from Dr. Burant. Mice were anaesthetized with isoflurane, the gastrocnemius muscle dissected and flash frozen with tongs cooled with liquid N2. They have been stored in cryovials in our -80 freezer for 2 months.
Institute
University of Michigan
DepartmentBiomedical Research Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-03-26
Num Groups2
Total Subjects14
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M86S3T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000252
Project DOI:doi: 10.21228/M86S3T
Project Title:Muscle Clock knock out metabolic changes
Project Summary:Inducible KO of Bmal1 in skeletal muscle; we see transcriptional changes suggesting significant effects in TCA/substrate metabolism
Institute:University of Kentucky
Department:Physiology
Laboratory:Esser Lab
Last Name:Esser
First Name:Karyn
Address:Lexington, KY
Email:karyn.esser@uky.edu
Phone:859-323-8107

Subject:

Subject ID:SU000333
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id muscle Bmal1
SA014085S00017952Bmal1 negative
SA014086S00017951Bmal1 negative
SA014087S00017953Bmal1 negative
SA014088S00017954Bmal1 negative
SA014089S00017955Bmal1 negative
SA014090S00017950Bmal1 negative
SA014091S00017949Bmal1 negative
SA014092S00017944Bmal1 positive
SA014093S00017943Bmal1 positive
SA014094S00017945Bmal1 positive
SA014095S00017946Bmal1 positive
SA014096S00017948Bmal1 positive
SA014097S00017947Bmal1 positive
SA014098S00017942Bmal1 positive
Showing results 1 to 14 of 14

Collection:

Collection ID:CO000327
Collection Summary:None
Sample Type:Muscle, Skeletal

Treatment:

Treatment ID:TR000347
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000341
Sampleprep Summary:None
Sampleprep Protocol Filename:Glycolysis-TCA-nucleotides-20150324.docx

Combined analysis:

Analysis ID AN000499
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode NEGATIVE
Units µM

Chromatography:

Chromatography ID:CH000353
Methods Filename:QTOF-002-HILIC-35min-1mm_bottompheater.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000437
Analysis ID:AN000499
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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