Summary of Study ST000019

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000018. The data can be accessed directly via it's Project DOI: 10.21228/M8Z59Q This work is supported by NIH grant, U2C- DK119886.


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Study IDST000019
Study TitleDetermine purity and quality of IROA labelled glucose
Study Type NMR and MS analysis
Study SummaryWe demonstrate the global metabolic analysis ofCaenorhabditis elegansstress responses using a mass-spectrometry-based technique called isotopic ratio outlier analysis (IROA). In an IROA protocol, control and experimental samples are isotopically labeled with 95 and 5%13C, and the two sample populations are mixed together for uniform extraction, sample preparation, and LC-MS analysis.To illustrate the utility of IROA for global metabolomics, we exposed wild-type (N2) worms to a heat shock (30 min heat shock at 33 C), which causes significant, widespread changes in metabolism. We collected and analyzed material from the exometabolome (all material that worms release in the supernatant) and the endometabolome (homogenized total extracts from the worm bodies).
University of Florida
DepartmentBiochemistry & Molecular Biology
Last NameStupp
First NameGregory
Submit Date2013-12-10
Study CommentsDetermine purity and quality of IROA labelled glucose
Raw Data AvailableYes
Raw Data File Type(s)ser
Uploaded File Size90 M
Analysis Type DetailNMR
Release Date2014-03-05
Release Version1
Gregory Stupp Gregory Stupp application/zip

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Combined analysis:

Analysis ID AN000038
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Accela 1250
Column Thermo Scientific Gold aQ
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap