Summary of Study ST002778

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001733. The data can be accessed directly via it's Project DOI: 10.21228/M8PX3J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002778
Study TitleCell Lineage-Guided Microanalytical Mass Spectrometry Reveals Increased Energy Metabolism and Reactive Oxygen Species in the Vertebrate Organizer
Study SummaryWe performed targeted metabolomic analysis on the Spemann-Mangold Organizer (SMO) tissue in the frog (Xenopus laevis) and the remainder of dissected embryos (RE). Metabolites were extracted from the dissected tissues, reconstituted, and analyzed using liquid chromatography (LC) electrospray ionization (ESI) mass spectrometry (MS). The targeted metabolite measurements were performed on a trapped ion mobility time-of-flight mass spectrometer (timsTOF PRO, Bruker).
Institute
University of Maryland
DepartmentChemistry & Biochemistry
Last NameNemes
First NamePeter
Address8051 Regents Drive, College Park, MD 20742, USA
Emailnemes@umd.edu
Phone3014050373
Submit Date2023-07-03
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2024-01-10
Release Version1
Peter Nemes Peter Nemes
https://dx.doi.org/10.21228/M8PX3J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004522 AN004523 AN004524
Analysis type MS MS MS
Chromatography type Ion exchange Ion exchange HILIC
Chromatography system Waters ACQUITY I-Class Waters Acquity I-Class Waters Acquity I-Class
Column Waters Atlantis Premier BEH C18 AX (100 x 2.1mm, 1.7um) Waters Atlantis Premier BEH C18 AX (100 x 2.1mm, 1.7um) ACQUITY UPLC BEH amide (100 x 1mm, 1.7um)
MS Type ESI ESI ESI
MS instrument type QTOF QTOF QTOF
MS instrument name Bruker timsTOF PRO Bruker timsTOF PRO Bruker timsTOF PRO
Ion Mode NEGATIVE NEGATIVE POSITIVE
Units Counts Counts Counts
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