Summary of Study ST000003

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000003. The data can be accessed directly via it's Project DOI: 10.21228/M8RP44 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000003
Study TitleMetabolomic analysis of mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells
Study TypeCell Type Comparison
Study Summarymouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells were compared via metabolomic analysis
University of California, Davis
DepartmentGenome Center
LaboratoryFiehn Laboratory
Last NameMeissen
First NameJohn
Address451 Health Sci Drive, Davis, CA 95616
Submit Date2013-01-16
Num Groups3
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size5.3 G
Analysis Type DetailLC-MS
Release Date2013-02-15
Release Version1
John Meissen John Meissen application/zip

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Combined analysis:

Analysis ID AN000003
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1290 Infinity
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Units Peak height


Chromatography ID:CH000003
Chromatography Summary:Untargeted HILIC Method
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Pressure:300 Bar (Max)
Column Temperature:40
Flow Gradient:0-4 min - 0% B, 4-12 min - Gradient to 45% B, 12-20 min - 45% B
Flow Rate:0.25 mL/min
Injection Temperature:4 C
Sample Injection:5 uL
Solvent A:100% water; 0.2% acetic acid; 5 mM ammonium acetate
Solvent B:90% acetonitrile/10% water; 0.2% acetic acid; 5 mM ammonium acetate
Analytical Time:20 min
Chromatography Type:HILIC