Summary of Study ST000529
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000388. The data can be accessed directly via it's Project DOI: 10.21228/M8WP4C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000529 |
Study Title | Regulation of Metabolism by LSR (part II) |
Study Type | Broad spectrum, reverse phase LCMS metabolomics (Negative ion mode) |
Study Summary | Our aim is to identify the LSR-driven metabolomics profile of breast cancer cells in lean and obesogenic environments. Breast cancer cell models with high or undetectable levels of LSR, including drug resistance models, were cultured in lean and obesogenic environments and comprehensive metabolomics profiling, including lipidomics-focused sub-analyses were performed. The metabolomics analyses using both approaches will help us determine if LSR enhances aggressive breast cancer phenotypes via modulation of cellular bioenergetic metabolism, ultimately contributing to poor patient outcome. |
Institute | University of North Carolina |
Department | Systems and Translational Sciences |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2016-12-30 |
Num Groups | 4 |
Total Subjects | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | LC-MS |
Release Date | 2018-02-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000807 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Waters Synapt G2 Si QTOF |
Ion Mode | NEGATIVE |
Units | m/z |
Chromatography:
Chromatography ID: | CH000582 |
Chromatography Summary: | Reversed-Phase Gradient Seperation |
Methods ID: | RTI-RCMRC-RP |
Methods Filename: | RTI-RCMRC-RP |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 50 °C |
Flow Rate: | 0.4 mL/min |
Internal Standard: | L-Tryptophan-d5 |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Analytical Time: | 22 min |
Weak Wash Solvent Name: | 10% MeOH |
Weak Wash Volume: | 1000 µL |
Strong Wash Solvent Name: | 80% MeOH |
Strong Wash Volume: | 1000 µL |
Target Sample Temperature: | 8 °C |
Sample Loop Size: | 10 µL |
Sample Syringe Size: | 100 µL |
Randomization Order: | Yes |
Chromatography Type: | Reversed phase |