Summary of Study ST000657
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000462. The data can be accessed directly via it's Project DOI: 10.21228/M8BG7G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000657 |
| Study Title | mTOR regulates metabolic adaptation of APCs in the lung microenvironment and controls the outcome of allergic inflammation |
| Study Summary | Antigen presenting cells (APCs) occupy diverse anatomical tissues, but their tissue-restricted homeostasis remains poorly understood. Here, working in mouse models of inflammation, we found that mTOR-dependent metabolic adaptation was required at discrete locations. mTOR was dispensable for DC homeostasis in secondary lymphoid tissues, but necessary to regulate cellular metabolism and accumulation of CD103+ DCs and alveolar macrophages in lung. Moreover, whilst numbers of mTOR-deficient lung CD11b+ DCs were not changed, they were metabolically reprogrammed to skew allergic inflammation from eosinophilic Th2 to neutrophilic Th17 polarity. The mechanism for this change was independent of translational control, but dependent on inflammatory DC which produced IL-23 and increased fatty acid oxidation. mTOR therefore mediates metabolic adaptation of APCs in distinct tissues, influencing the immunological character of allergic inflammation. |
| Institute | Emory University |
| Last Name | Li; Gardinassi |
| First Name | Shuzhao; Luiz |
| Address | Emory Woodruff Memorial Research Building, 1639 Pierce Dr NE, Atlanta, GA 30322 |
| shuzhao.li@gmail.com; luiz.gardinassi@emory.edu | |
| Phone | 404-712-2988 or 404-727-5091 |
| Submit Date | 2017-06-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-07-17 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001003 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | au |
Chromatography:
| Chromatography ID: | CH000720 |
| Chromatography Summary: | Untargeted analysis with HILIC positive mode. |
| Methods Filename: | Metab_HFQE_Setup.docx |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| Column Temperature: | 30 |
| Flow Gradient: | 0-2min, 10%A-80%B-10%C; 2-7min, 80%A-10%B-10%C; 7-10min, 80%A-10%B-10%C. |
| Flow Rate: | .350 ul/min |
| Internal Standard: | internal standards: [13C6]-D-glucose, [15N]-indole, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine |
| Sample Injection: | 10ul |
| Sampling Cone: | HESI probe with S-lens combination for ESI |
| Solvent A: | 100% water(A), 100% acetonitrile(B), 100% water; 2% formic acid(C) |
| Solvent B: | 100% water(A), 100% acetonitrile(B), 100% water; 2% formic acid(C) |
| Analytical Time: | 10min |
| Weak Wash Solvent Name: | water with 10% methanol |
| Chromatography Type: | HILIC |
