Summary of Study ST001220
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000819. The data can be accessed directly via it's Project DOI: 10.21228/M8TD5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001220 |
Study Title | Effects of cadmium exposure on the bumble bee metabolome |
Study Summary | We exposed worker bumble bees to cadmium chloride or control through diet. We then used LCMS untargeted metabolomics on pools of 3 bees each. |
Institute | University of California, Riverside |
Last Name | Rothman |
First Name | Jason |
Address | 900 University Ave., Riverside, CA, 91766, USA |
jroth002@ucr.edu | |
Phone | 9518275817 |
Submit Date | 2019-07-16 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | GC-MS |
Release Date | 2019-09-23 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002033 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity I-Class |
Column | Waters Acquity CSH Phenyl-Hexyl (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Waters Synapt G2 Si QTOF |
Ion Mode | POSITIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH001473 |
Chromatography Summary: | We used a Synapt G2-Si quadrupole time-of-flight mass spectrometer (Waters, Milford, MA) coupled to an I-class UPLC system (Waters) for LC-MS analyses in the UC Riverside Metabolomics Core Facility. We carried out separations on a CSH phenyl-hexyl column (2.1 x 100 mm, 1.7 µM) (Waters, Milford, MA), with the following mobile phases: A. Water with 0.1% formic acid and B. Acetonitrile with 0.1% formic acid at a flow rate of 250 µL/min at 40° C. We injected 2 µL of sample extract, and the gradient was as follows: 0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B. |
Methods Filename: | jroth002_LC_method.docx |
Instrument Name: | Waters Acquity I-Class |
Column Name: | Waters Acquity CSH Phenyl-Hexyl (100 x 2.1mm,1.7um) |
Column Temperature: | 40 |
Flow Gradient: | 0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B. |
Flow Rate: | 250 µL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |