Summary of Study ST001252

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000839. The data can be accessed directly via it's Project DOI: 10.21228/M87H7W This work is supported by NIH grant, U2C- DK119886.


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Study IDST001252
Study TitleEicosanoid profiles of dermal fibroblasts (part-I)
Study SummaryThe sphingolipid, ceramide-1-phosphate (C1P), directly binds and activates Group IVA cytosolic phospholipase A2 (cPLA2) to generate eicosanoids. Due to the role of eicosanoids in wound healing, we choose to use our novel genetic mouse model expressing cPLA2 with an ablated C1P interaction site (KI) to examine the cPLA2/C1P interaction in wound healing. Wound closure rate was not affected, but wound maturation was enhanced by loss of the C1P/cPLA2α interaction based on the following findings. Wounds in KI mice displayed: i) increased infiltration of dermal fibroblasts into the wound environment; ii) increased wound tensile strength; and iii) higher Type I/Type III collagen ratios. These findings were recapitulated in vitro as primary dermal fibroblasts (pDFs) from KI mice showed significantly increased collagen deposition and migration velocity compared to WT and KO pDFs. Additionally, the KI showed an altered eicosanoid profile of reduced pro-inflammatory prostaglandins (PGE2) and increased levels of specific HETE species (5-HETE). Elevated 5-HETE levels promoted increased dermal fibroblast migration and collagen deposition. This “gain of function” role for the mutant cPLA2 was also linked to differential cellular localization of cPLA2α and 5-HETE biosynthetic factors. These studies demonstrate regulation of key biological mechanisms by a defined protein:lipid interaction in vivo and provide new insights into cPLA2 function.
University of South Florida
Last NameChalfant
First NameCharles
Address4202 E Fowler Ave, Tampa, FL 33620
Submit Date2019-09-22
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailMALDI-MS
Release Date2019-10-11
Release Version1
Charles Chalfant Charles Chalfant application/zip

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Combined analysis:

Analysis ID AN002078
Analysis type MS
Chromatography type Reversed phase
Chromatography system Shimadzu Nexera X2
Column Acentis Express C18 column
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap
Units pmol of lipid


Chromatography ID:CH001515
Instrument Name:Shimadzu Nexera X2
Column Name:Acentis Express C18 column
Chromatography Type:Reversed phase