Summary of Study ST001311
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000890. The data can be accessed directly via it's Project DOI: 10.21228/M8ND7K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001311 |
| Study Title | Lipid expression in liver after early lifer exposure to an endocrine disruptor at 70 days postnatal (part-II) |
| Study Type | Lipid expression after chemical exposure versus control |
| Study Summary | Our early-life environment has a profound influence on developing organs that impact metabolic function and determines disease susceptibility across the life-course. Using a rat model for exposure to an endocrine disrupting chemical (EDC), we show that early-life exposure causes metabolic dysfunction in adulthood and reprograms histone marks in the developing liver to accelerate acquisition of an adult epigenomic signature. This epigenomic reprogramming persists long after the initial exposure, but many reprogrammed genes remain transcriptionally silent with their impact on metabolism not revealed until a later life exposure to a Western-style diet. Diet-dependent metabolic disruption was largely driven by reprogramming of the Early Growth Response 1 (EGR1) transcriptome and production of metabolites in pathways linked to cholesterol, lipid and one-carbon metabolism. These findings demonstrate the importance of epigenome:environment interactions, which early in life accelerate epigenomic aging, and later in adulthood unlock metabolically restricted epigenetic reprogramming to drive metabolic dysfunction. |
| Institute | Baylor College of Medicine |
| Last Name | Walker |
| First Name | Cheryl |
| Address | 1 Baylor Plaza, Houston, TX, 77030, USA |
| Cheryl.walker@bcm.edu | |
| Phone | 713-798-8219 |
| Submit Date | 2020-01-24 |
| Num Groups | 2 |
| Total Subjects | 10 |
| Num Males | 10 |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-03-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002182 | AN002183 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu Nexera-x2 | Shimadzu Nexera-x2 |
| Column | 1.8 μm particle 50 × 2.1 mm | 1.8 μm particle 50 × 2.1 mm |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600 TripleTOF | ABI Sciex 5600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak intensity | peak intensity |
Chromatography:
| Chromatography ID: | CH001597 |
| Instrument Name: | Shimadzu Nexera-x2 |
| Column Name: | 1.8 μm particle 50 × 2.1 mm |
| Column Temperature: | 55 |
| Flow Gradient: | linear gradient over a 20 min total run time, with 60% solvent A and 40% solvent B gradient in the first 10 minutes, then the gradient was ramped in a linear fashion to 100% solvent B which was maintained for 7 minutes. After that the system was switched back to 60% solvent B and 40% solvent A for 3 minutes. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001598 |
| Instrument Name: | Shimadzu Nexera-x2 |
| Column Name: | 1.8 μm particle 50 × 2.1 mm |
| Column Temperature: | 55 |
| Flow Gradient: | linear gradient over a 20 min total run time, with 60% solvent A and 40% solvent B gradient in the first 10 minutes, then the gradient was ramped in a linear fashion to 100% solvent B which was maintained for 7 minutes. After that the system was switched back to 60% solvent B and 40% solvent A for 3 minutes. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
