Summary of Study ST001362
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000932. The data can be accessed directly via it's Project DOI: 10.21228/M8710D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001362 |
| Study Title | California mouse fecal metabolite analysis |
| Study Type | Effect of BPA and genistein exposure on the fecal metabolome |
| Study Summary | Xenoestrogens are found in plant products, such as genistein (GEN), or industrial chemicals, such as bisphenol A (BPA), present in consumer products that are also pervasive in the environment. Early exposure to such endocrine disrupting chemicals (EDC) may affect neural development by inducing direct neural effects and/or through the microbiome-gut-brain axis. To test this hypothesis, California mice (Peromyscus californicus) offspring were exposed through the maternal diet to GEN (250 mg/kg feed weight) or BPA (5 mg/kg feed weight, low dose- LD and 50 mg/kg, upper dose-UD), and dams were placed on these diets two weeks prior to breeding, throughout gestation, and lactation. Various behaviors, gut microbiome, and fecal metabolome were assessed starting at 90 days of age. The LD but not UD of BPA resulted in individuals spending more time engaging in repetitive behaviors. GEN exposed individuals were more likely to exhibit such behaviors and showed socio-communicative disturbances. BPA and GEN exposed females had increased number of metabolites involved in carbohydrate metabolism and synthesis.. Males exposed to BPA or GEN showed alterations in lysine degradation and phenylalanine and tyrosine metabolism. Current findingsindicate cause for concern that developmental exposure to BPA or GEN might affect the microbiome-gut-brain axis. |
| Institute | University of Missouri |
| Department | MU Metabolomics Center |
| Last Name | Sarma |
| First Name | Saurav |
| Address | 1201 Rollins street, 243 Bond Life Science Center, University of Missouri, Columbia, MO 65211, USA |
| sarmas@missouri.edu | |
| Phone | 3366713357 |
| Submit Date | 2020-04-12 |
| Num Groups | 8 |
| Total Subjects | 78 |
| Num Males | 37 |
| Num Females | 41 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | baf |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2020-05-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002266 | AN002267 | AN002268 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | GC | GC |
| Chromatography system | Waters Acquity I-Class | Agilent 6890N | Agilent 6890N |
| Column | Waters Acquity BEH C18 (150 x 2mm,1.7um) | DB-5MS (J&W Scientific) 60m | DB-5MS (J&W Scientific) 60m |
| MS Type | ESI | EI | EI |
| MS instrument type | QTOF | Single quadrupole | Single quadrupole |
| MS instrument name | Bruker maXis Impact qTOF | Agilent 5973 | Agilent 5973 |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE |
| Units | intensity | intensity | intensity |
Chromatography:
| Chromatography ID: | CH001665 |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters Acquity BEH C18 (150 x 2mm,1.7um) |
| Column Pressure: | >11000 psi |
| Column Temperature: | 60 |
| Flow Gradient: | Solvent B (acetonitrile) was increased from 5% to 70% over 30 min, then to 95% over 3 min, held at 95% for 3 min, then returned to 5% for equilibrium |
| Flow Rate: | 0.560 ml/min |
| Internal Standard: | Umbelliferon |
| Retention Time: | 1 to 35 min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 40 min |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001666 |
| Chromatography Summary: | 5:1 split for GCMS-polar injection with 1uL injection volume. |
| Instrument Name: | Agilent 6890N |
| Column Name: | DB-5MS (J&W Scientific) 60m |
| Column Pressure: | variable |
| Column Temperature: | variable between 80-315 |
| Flow Rate: | 1mL/Min of Helium gas |
| Internal Standard: | Ribitol |
| Retention Time: | 9 to 61 min |
| Sample Injection: | 5:1 split injection with 1uL injection volume |
| Analytical Time: | 61 min |
| Chromatography Type: | GC |
| Chromatography ID: | CH001667 |
| Chromatography Summary: | 1:1 split for GCMS-nonpolar injection with 1uL injection volume. |
| Instrument Name: | Agilent 6890N |
| Column Name: | DB-5MS (J&W Scientific) 60m |
| Column Pressure: | variable |
| Column Temperature: | variable between 80-315 |
| Flow Rate: | 1mL/Min of Helium gas |
| Internal Standard: | Docosanol |
| Retention Time: | 9 to 61 min |
| Sample Injection: | 1:1 split injection with 1uL injection volume |
| Analytical Time: | 61 min |
| Chromatography Type: | GC |
