Summary of Study ST001812
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001145. The data can be accessed directly via it's Project DOI: 10.21228/M8Q12H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001812 |
| Study Title | Evidence that class I glutamine amidotransferase, GAT1_2.1, acts as a glutaminase in roots of Arabidopsis thaliana (part II) |
| Study Type | Targeted Metabolite Quantification |
| Study Summary | In this study, we used Arabidopsis root extracts, spiked with amide nitrogen labeled (15N1) Glutamine and a purified recombinant protein, both full length and glutaminase domain only versions, to determine the amido group acceptor, if any, in the glutamine amidotransferase reaction. |
| Institute | Agriculture and Agri-Food Canada |
| Department | London Research and Development Centre |
| Laboratory | Frederic Marsolais |
| Last Name | Kambhampati |
| First Name | Shrikaar |
| Address | 1391 Sandford St, London, ON N5V 4T3, Canada |
| shrikaar.k@gmail.com | |
| Phone | 3144025550 |
| Submit Date | 2021-06-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-06-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002937 | AN002938 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Intensity | Intensity |
Chromatography:
| Chromatography ID: | CH002176 |
| Methods Filename: | Enzyme_spike_analysis.pdf |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| Column Temperature: | 35 |
| Flow Rate: | 0.3 mL min-1 |
| Internal Standard: | 13C6 Phenylalanine |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 4 |
| Solvent B: | 90% acetonitrile/10% water; 0.1% acetic acid |
| Chromatography Type: | HILIC |
