Summary of Study ST001816
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001149. The data can be accessed directly via it's Project DOI: 10.21228/M8611G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001816 |
Study Title | Quantification of PIPs species in mouse tissues. |
Study Summary | Phosphoinositides (PIPs) species, differing in phosphorylation at hydroxyls of the inositol head group, play roles in various cellular events. Here we developed a supercritical fluid chromatography-mass spectrometry (SFC-MS) method that allows us to quantify molecular species of all seven PIP regioisomers in culture cells and tissues. Using this methodology, we quantified PIPs species in 13 mouse tissues. |
Institute | Grad Sch of Pharmaceut Sci, Univ of Tokyo |
Last Name | Kono |
First Name | Nozomu |
Address | Hongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan |
nozomu@mol.f.u-tokyo.ac.jp | |
Phone | +81-3-5841-4723 |
Submit Date | 2021-06-01 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2022-05-04 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002948 |
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Analysis type | MS |
Chromatography type | Unspecified |
Chromatography system | Shimadzu Nexera UC |
Column | ULTRON AF-HILIC-CD (250 × 2.1 mm,5.0um) Shinwa Chemicalstries |
MS Type | ESI |
MS instrument type | Ion trap |
MS instrument name | ABI SCIEX 4500 QTrap |
Ion Mode | POSITIVE |
Units | pmol/mg tissue |
Chromatography:
Chromatography ID: | CH002183 |
Instrument Name: | Shimadzu Nexera UC |
Column Name: | ULTRON AF-HILIC-CD (250 × 2.1 mm,5.0um) Shinwa Chemicalstries |
Column Temperature: | 4℃ |
Flow Gradient: | 0–16 min: 5% B→20% B; 16.01–18 min: 40% B; 18.01–22 min: 5% B |
Flow Rate: | 1.5 ml/min |
Solvent A: | supercritical carbon dioxide (SCCO2) |
Solvent B: | 2.5% water/97.5% methanol; 0.1% formic acid |
Chromatography Type: | Unspecified |