Summary of Study ST001893
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001192. The data can be accessed directly via it's Project DOI: 10.21228/M8MX3X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001893 |
| Study Title | Involvement of Mieap in Cardiolipin metabolism (part I) |
| Study Summary | Quantitative assessment of total cardiolipin (CL) and comparison of CL species conducted with A549 (Ad-Mieap infected vs. non-infected) and LS174T cells (LS174T-cont vs. Mieap-KD). The A549 cells were harvested 24 hr after infection with Ad-Mieap and were compared with non-infected cells by mass spectrometric analysis. The LS174T-cont and Mieap-KD cells incubated under a normal condition were harvested and subjected to mass spectrometric analysis. |
| Institute | National Cancer Center Japan Research Institute |
| Last Name | Ikari |
| First Name | Naoki |
| Address | 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan |
| nikari@ncc.go.jp | |
| Phone | +81-3-3542-2511 |
| Submit Date | 2021-07-30 |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-09 |
| Release Version | 1 |
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Combined analysis:
| Analysis ID | AN003074 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | UltiMate 3000 (Thermo Fisher Scientific) |
| Column | Waters XBridge C18 (150 x 1.0mm,3.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | NEGATIVE |
| Units | pmol/10,000,000 cells |
Chromatography:
| Chromatography ID: | CH002274 |
| Chromatography Summary: | An aliquot of the organic phase was added to an equal volume of methanol before being loaded onto a DEAE-cellulose column (Wako Chemical) pre-equilibrated with chloroform. After successive washes with chloroform/methanol (1:1, v/v), acidic phospholipids were eluted with chloroform/methanol/HCl/water (12:12:1:1, v/v), followed by evaporation to dryness to yield a residue was soluble in methanol. |
| Instrument Name: | UltiMate 3000 (Thermo Fisher Scientific) |
| Column Name: | Waters XBridge C18 (150 x 1.0mm,3.5um) |
| Column Temperature: | 40 |
| Flow Gradient: | ratios of 60%/40% (0 min), 40%/60% (1 min), 20%/80% (9 min), 5%/95% (11-30 min), 95%/5% (31-35 min) and 60%/40% (45 min) |
| Flow Rate: | 25ul/min |
| Solvent A: | 50% isopropanol/10% methanol/40% water; 5 mM ammonium formate;0.05% ammonium hydroxide |
| Solvent B: | 100% isopropanol; 5 mM ammonium formate; 0.05% ammonium hydroxide |
| Chromatography Type: | Reversed phase |
