Summary of Study ST002278

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001459. The data can be accessed directly via it's Project DOI: 10.21228/M84H7Z This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002278
Study TitleModulation of blood metabolites by dietary β–glucan in rainbow trout (Oncorhynchus mykiss) - Serum lipid concentrations
Study SummaryMeasurement of changes in serum lipid levels using targeted mass spectrometry
Institute
Helmholtz Centre for Environmental Research
Last NameHaange
First NameSven
AddressPermoserstrasse 1, Leipzig, Saxony, 04318, Germany
Emailsven.haange@ufz.de
Phone0049 341 2351099
Submit Date2022-08-19
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-09-22
Release Version1
Sven Haange Sven Haange
https://dx.doi.org/10.21228/M84H7Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003722
Analysis type MS
Chromatography type Reversed phase
Chromatography system UPLC (Waters Acquity, Waters Corporation, Milford, USA)
Column Agilent, Zorbax Eclipse XDB C18, 3.0 x 100 mm, 3.5 µM, Agilent Waldbronn, Germany
MS Type ESI
MS instrument type QTRAP
MS instrument name ABI Sciex 5500 QTrap
Ion Mode POSITIVE
Units µM

Chromatography:

Chromatography ID:CH002756
Chromatography Summary:LC - Instrument Parameters AbsoluteIDQ® p180 Kit (Biocrates Life Science AG, Innsbruck, Austria) System: UPLC (Waters Acquity, Waters Corporation, Milford, USA) Column: Agilent, Zorbax Eclipse XDB C18, 3.0 x 100 mm, 3.5 µM, Agilent Waldbronn, Germany Precolumn: Security Guard, Phenomenex, C18, 4 x 3 mm; Phenomenex, Aschaffenburg, Germany Materials: Water MiliQ, Methanol (Merck KGaA, Darmstadt, Germany, hypergrade for LC-MS) Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Ammonium Acetate (Honeywell - Fluka, Seelze, Germany) Formic Acid (Honeywell, Fluka, Seelze, Germany) Running Solvent: 5mM ammonium acetat in methanol FIA: B: Mix running solvent and MiliQ water 1:1 Gradient Table FIA Time (min) Flow Rate (mL/min) %A %B Curve Initial 0.030 0.0 100.0 Initial 1.60 0.030 0.0 100.0 6 2.40 0.200 0.0 100.0 6 2.80 0.200 0.0 100.0 6 3.00 0.030 0.0 100.0 6
Methods Filename:LC_parameters_metabolites.pdf
Instrument Name:UPLC (Waters Acquity, Waters Corporation, Milford, USA)
Column Name:Agilent, Zorbax Eclipse XDB C18, 3.0 x 100 mm, 3.5 µM, Agilent Waldbronn, Germany
Chromatography Type:Reversed phase
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