Summary of Study ST002537

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001633. The data can be accessed directly via it's Project DOI: 10.21228/M8MM8Q This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002537
Study TitleOsmoprotectants play a major role in the Portulaca oleracea resistance to high levels of salinity stress - Insights from a metabolomics and proteomics integrated approach
Study SummaryPurslane is an invasive plant and is considered the eighth most common weed in the world. Because of that, its outdoor production in extensive areas faces several concerns. Kong & Zheng (2014) evaluated the potential of producing purslane in a hydroponic system, generating approximately 5.75 kg of fresh matter per m2 per month, which might yield 57.5 tons/hectare/year if cultivated in a bimestrial regime. The high productivity of purslane, when grown in controlled-environment agriculture, can open many opportunities for the purslane industry, even in the context of biosaline agriculture. Building up a robust multi-omics database on the response of purslane to salt stress and the subsequent study of it via an MOI analysis can create the basis for a future system biology approach to decode the genetics behind its resistance to salinity stress. The present study is a second step in building a robust database on the morpho-physiological and molecular responses of Portulaca oleracea L. to salinity stress and its subsequent use in attempting to decode the genetics behind its resistance to this abiotic stress. After reporting on the characterization of the morpho-physiological responses of young purslane plants to such stress using a robust salinization protocol, here we report a study on adult purslane plants through the characterization of the untargeted metabolome and proteome profiles on the leaves and roots of this halophyte species submitted to very high salinity stress, and the consequent use of single- and multi-omics analysis strategies to study it.
Embrapa Agroenergy
Last NameSouza Júnior
First NameManoel Teixeira
AddressParque Estacao Biologica final Asa Norte Brasília DF 70770-901 BR, PQEB, sn - Asa Norte, DF
Phone+55 (61) 3448-4246
Submit Date2023-04-03
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-06-06
Release Version1
Manoel Teixeira Souza Júnior Manoel Teixeira Souza Júnior application/zip

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Combined analysis:

Analysis ID AN004175 AN004176
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu Nexera X2 Shimadzu Nexera X2
Column Waters ACQUITY UPLC BEH C8 (150 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH C8 (150 x 2.1mm,1.7um)
MS instrument type QTOF QTOF
MS instrument name Bruker maXis Impact qTOF Bruker maXis Impact qTOF
Units m/z m/z


Chromatography ID:CH003093
Instrument Name:Shimadzu Nexera X2
Column Name:Waters ACQUITY UPLC BEH C8 (150 x 2.1mm,1.7um)
Column Temperature:40 °C
Flow Gradient:isocratic at the start (0 - 0.5 min) with 4% of B solvent, then at linear gradient (0.5 – 10 min) with 34% B and (10 – 15 min) with 100% B, and finally isocratic (15 – 18 min) with 100% B
Flow Rate:400 μL min−1
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase