Summary of Study ST002559
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001651. The data can be accessed directly via it's Project DOI: 10.21228/M8971G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002559 |
| Study Title | The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (VCSIP Cohort) |
| Study Type | Prospective Cohort Study |
| Study Summary | Maternal smoking during pregnancy negatively effects fetal lung development and causes lifelong decreases in offspring pulmonary function and offspring respiratory health including increased risk of childhood asthma development. The VCSIP cohort was created from 2 RCTs to determine if vitamin C supplementation to pregnant smokers could ameliorate some of the negative effects of maternal smoking during pregnancy on offspring lung health (JAMA 311:2074-2082, 2014; JAMA Pediatr 177:16-24, 2023). Both pre- and postnatal tobacco exposures have been carefully monitored and extensive respiratory phenotyping on the offspring have been performed. Please contact Cindy McEvoy at Mcevoyc@ohsu.edu for questions related to subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) Program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. VCSIP is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539 |
| Institute | Oregon Health and Science University |
| Department | Division of Neuroscience |
| Last Name | Spindel |
| First Name | Eliot |
| Address | 3181 SW Sam Jackson Park Road Portland, OR 97239-3098 |
| spindele@ohsu.edu | |
| Phone | 503-346-5524 |
| Submit Date | 2023-04-13 |
| Total Subjects | 200 |
| Study Comments | HHEAR Project EM20-0011, ECHO Project EC0376 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Chear Study | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-04-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004218 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE |
| Units | m/z |
Chromatography:
| Chromatography ID: | CH003128 |
| Chromatography Summary: | Reverse phase |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Pressure: | 6000-10000 psi |
| Column Temperature: | 50℃ |
| Flow Gradient: | Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5 |
| Flow Rate: | 0.4 mL/min |
| Injection Temperature: | 8oC |
| Internal Standard: | Tryptophan-d5 |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Analytical Time: | 22 min |
| Weak Wash Solvent Name: | 10% methanol/90% water; 0.1% formic acid |
| Strong Wash Solvent Name: | 75% 2-Propanol/25% Water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
