Summary of Study ST002751
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002751 |
Study Title | Biomolecular condensates create phospholipid-enriched microenvironments (Part 5) |
Study Type | Metabolomes of mouse liver |
Study Summary | In this study we used LC-MS and MS/MS to characterize the metabolomes of the input mouse liver metabolites used in the first two studies of this submission. |
Institute | Cornell University |
Department | Department of Pharmacology |
Laboratory | Dr. Samie Jaffrey |
Last Name | Dumelie |
First Name | Jason |
Address | 1300 York Ave, LC-524, New York City, NY |
srj2003@med.cornell.edu | |
Phone | 6465690174 |
Submit Date | 2023-06-14 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzdata.xml |
Analysis Type Detail | LC-MS |
Release Date | 2023-07-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004463 | AN004464 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Normal phase | Normal phase |
Chromatography system | Agilent Model 1290 Infinity II liquid chromatography system | Agilent Model 1290 Infinity II liquid chromatography system |
Column | Cogent Diamond Hydride (150 × 2.1 mm, 4um) | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Ion abundance (peak area) | Ion abundance (peak area) |
Chromatography:
Chromatography ID: | CH003350 |
Chromatography Summary: | Tissue extracts were analyzed by LC/MS as described previously, using a platform comprised of an Agilent Model 1290 Infinity II liquid chromatography system coupled to an Agilent 6550 iFunnel time-of-flight MS analyzer. Chromatography of metabolites utilized aqueous normal phase (ANP) chromatography on a Diamond Hydride column (Microsolv). Mobile phases consisted of: (A) 50% isopropanol, containing 0.025% acetic acid, and (B) 90% acetonitrile containing 5 mM ammonium acetate. To eliminate the interference of metal ions on chromatographic peak integrity and electrospray ionization, EDTA was added to the mobile phase at a final concentration of 5 µM. The following gradient was applied: 0-1.0 min, 99% B; 1.0-15.0 min, to 20% B; 15.0 to 29.0, 0% B; 29.1 to 37min, 99% B. |
Instrument Name: | Agilent Model 1290 Infinity II liquid chromatography system |
Column Name: | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
Column Temperature: | 26 |
Flow Gradient: | The following gradient was applied: 0-1.0 min, 99% B; 1.0-15.0 min, to 20% B; 15.0 to 29.0, 0% B; 29.1 to 37min, 99% B. |
Flow Rate: | 0.3 mL/min |
Solvent A: | 50% isopropanol/50% water; 0.025% acetic acid |
Solvent B: | 90% acetonitrile/10% water; 5 mM ammonium acetate |
Chromatography Type: | Normal phase |