Summary of Study ST002931
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001823. The data can be accessed directly via it's Project DOI: 10.21228/M82Q61 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002931 |
Study Title | Analyzing Metabolic Alterations in the Gut, Blood, and Brain of Mice Induced by Running Exercise Through Gas Chromatography Mass Spectrometry. |
Study Summary | Studying the metabolic impact of running exercise on the gut, blood, and specific brain regions like the hippocampus and brainstem is crucial for comprehending the broader health effects of physical activity. In our six-week study, we utilized a mouse model (C57BL/6 genotype) to investigate these metabolic changes. Employing gas chromatography coupled with mass spectrometry followed by metabolomics for a comprehensive analysis, our approach offers insights into how exercise influences metabolic processes, including brain function. Our findings hold the potential to shape more effective exercise strategies for enhancing overall health and cognitive function. |
Institute | University of Puerto Rico, School of Medicine |
Department | Biochemistry |
Last Name | Chorna |
First Name | Nataliya |
Address | University of Puerto Rico, Medical Sciences Campus, San Juan, PR 00935 |
nataliya.chorna@upr.edu | |
Phone | 787-758-2525 x 1640 |
Submit Date | 2023-10-14 |
Num Groups | 2 |
Total Subjects | 24 |
Num Males | 24 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2023-10-30 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004809 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Shimadzu GCMS/MS-TQ8050 |
Column | Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Triple quadrupole |
MS instrument name | Shimadzu GCMS/MS-TQ8050 |
Ion Mode | POSITIVE |
Units | intensity |
Chromatography:
Chromatography ID: | CH003634 |
Chromatography Summary: | 1 μL of each sample was injected in the GC-MS. Injection mode – split (15:1). The injection port temperature was set at 280°C, and helium was used as the carrier gas at a constant linear velocity of 39 cm/sec. Metabolites were separated using a GC temperature ramping program. The GC oven was programmed from 100°C to 280°C at a rate of 4°C/min. |
Methods Filename: | Chromatography.pdf |
Instrument Name: | Shimadzu GCMS/MS-TQ8050 |
Column Name: | Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um) |
Column Temperature: | 280°C |
Flow Gradient: | NA |
Flow Rate: | 4°C/min |
Solvent A: | NA |
Solvent B: | NA |
Chromatography Type: | GC |