Summary of Study ST000454
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000351. The data can be accessed directly via it's Project DOI: 10.21228/M8PC8X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000454 |
Study Title | Utilizing Metabolomics to Understand Novel Anti-Desmoid Tumor Drugs (part I) |
Study Summary | This pilot study will use broad spectrum metabolomics to study the tumorigenesis process of fibroblasts to desmoids by investigating paired desmoid and fibroblast cell lines, in addition to unaffected fibroblast cells. Additionally, this pilot study will explore the effects of two of the active drugs identified on the desmoid and fibroblast cells. |
Institute | RTI International |
Last Name | Mercier |
First Name | Kelly |
Address | 3040 E. Cornwallis Road |
kmercier@rti.org | |
Phone | 919-541-6396 |
Submit Date | 2016-08-31 |
Raw Data Available | Yes |
Raw Data File Type(s) | 1r |
Analysis Type Detail | NMR |
Release Date | 2018-02-07 |
Release Version | 1 |
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Collection:
Collection ID: | CO000469 |
Collection Summary: | An aliquot of the media following treatment was collected, and the remainder of the media was aspirated. The cells were washed with PBS twice, and quenched with 8mL ice-cold isotonic 0.9% (w/v) saline for 2 minutes. Total cellular content was then extracted with 1.7mL ice-cold acetonitrile/water (50:50, v/v) solution. Cell extracts were collected using a cell scraper and quickly transferred to MagNA Lyser Green Beads tubes (Roche, Indianapolis, USA) and stored in -80°C. Media was added to empty plates and incubated together with the cells for the duration of the experiment served as a blank. |
Sample Type: | Fibroblasts |