Summary of Study ST001426

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000978. The data can be accessed directly via it's Project DOI: 10.21228/M88Q44 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001426
Study TitleDependence of the Staphylococcal Volatilome Composition on Microbial Nutrition
Study TypeUntargeted MS
Study SummaryIntroduction: In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, and selection of growth medium will substantially influence staph growth rates, genetic integrity, pathogenicity, and metabolic capacity. Few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. Objectives: The objective of this study was to determine the influence of rich media composition on the chemical characteristics of the volatilomes of Staphylococcus aureus and S. epidermidis. Methods: S. aureus (ATCC 12600) and S. epidermidis (ATCC 12228) were cultured in triplicate in four rich complex media (BHI, LB, MHB, and TSB), and the volatile metabolites produced by each culture were analyzed using headspace solid-phase microextraction combined with comprehensive two-dimensional gas chromatography – time-of-flight mass spectrometry (HS-SPME-GC×GC-TOFMS). Results: When comparing the chemical compositions of the staph volatilomes produced in each medium, we observed few differences when the presence versus absence of volatiles were compared. However, when the relative abundances of volatiles were included in the analyses, we observed that culturing staph in media containing free glucose (BHI and TSB) resulted in volatilomes dominated by acids and esters (67%). The low-glucose media (LB and MHB) produced ketones in greatest relative abundances, but the volatilome compositions in these two media were highly dissimilar. Conclusion: The staphylococcal volatilome is strongly influenced by the nutritional composition of the growth medium, especially the availability of free glucose, which is much more evident when the relative abundances of the volatiles are analyzed, compared to the presence versus absence.
Arizona State University
DepartmentSchool of Life Sciences
LaboratoryHeather D. Bean Lab
Last NameBean, Ph.D.
First NameHeather
AddressPO Box 874501 Tempe, AZ 85287
Submit Date2020-07-13
Study CommentsStaphylococcus aureus (ATCC 12600) and Stpahylococcus epidermidis (ATCC 12228) grown in four complex media
PublicationsJenkins, C. L., H. D. Bean (2020). Influence of media on the differentiation of Staphylococcus spp. by volatile compounds. Journal of breath research 14, 016007 doi:10.1088/1752-7163/ab3e9d
Raw Data AvailableYes
Raw Data File Type(s)smp
Analysis Type DetailGC-MS
Release Date2020-07-30
Release Version1
Heather Bean Heather Bean
Ph.D. Ph.D. application/zip

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Collection ID:CO001495
Collection Summary:Staphylococcus aureus (ATCC 12600) and Staphylococcus epidermidis (ATCC 12228) were cultured in four filter-sterilized complex media for metabolomics analysis: Brain Heart Infusion broth (BHI; Bacto); lysogeny broth Lennox (LB; Fisher Scientific); Mueller Hinton broth (MHB; Difco); and Tryptic Soy broth (TSB; Bacto). Each species was prepared in biological triplicate. Uninoculated media control blanks were prepared in six replicates for BHI, LB, and MHB, and in triplicate for TSB, following the same procedures as the bacterial samples and processed in parallel. Samples were transferred to capped GC vials and stored at -20 °C for approximately two weeks prior to GC×GC-TOFMS analysis.
Collection Protocol Filename:CLJenkins_Collection_Protocol_Metadata.txt
Sample Type:Bacterial cells
Collection Frequency:Once, at the completion of 24 hour aerobic incubation at 37 C with orbital shaking