Summary of Study ST001635
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001044. The data can be accessed directly via it's Project DOI: 10.21228/M8RD7H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001635 |
| Study Title | Higher Dietary Carbohydrates Detrimentally Impact Obesity-Associated Breast Cancer Chemoresistance |
| Study Type | Untargeted UPLC-MS Metabolomics Analysis |
| Study Summary | This study is a part of an ongoing project entitled, “Higher Dietary Carbohydrates Detrimentally Impact Obesity-Associated Breast Cancer Chemoresistance.” (See project). Untargeted LCMS metabolomics analysis was performed to identify metabolic markers of nutritionally dependent and obesity-associated chemoresistance in female C57LB/6 mice fed either a high carbohydrate plus high fat (HCHF) diet or a high protein diet (HP). Mice were fed 15 weeks on either diet, then injected with a MMTV-Wnt-1 mouse mammary basal-like breast cancer cell line, for tumor formation up to 6 weeks. Three mice on either diet were treated for 24 hr with Doxorubicin or Saline as a vehicle control. All samples were analyzed in the positive mode for untargeted metabolomics analysis. Liver samples were selected for analysis because of an observation of fatty liver development in mice only on the HCHF diet after 15 weeks. Samples were extracted and analyzed by UPLS-MS analysis using Q-Exactive HFX mass spectrometer. |
| Institute | University of North Carolina at Chapel Hill |
| Department | Nutrition |
| Laboratory | Metabolomics and Exposome Laboratory, Nutrition Research Institute, UNC Chapel Hill |
| Last Name | Sumner |
| First Name | Susan |
| Address | 500 Laureate Way, Nutrition Research Institute, UNC Chapel Hill |
| susan_sumner@unc.edu | |
| Phone | (919) 622-4456 |
| Submit Date | 2020-12-18 |
| Num Groups | 6 |
| Total Subjects | 193 |
| Num Females | 175 |
| Study Comments | The number of groups includes the QC samples |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-12-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO001705 |
| Collection Summary: | See the project foe more details. Briefly, mice were euthanized and biospecimens were collected at the following study endpoints: 1-baseline (following 1-week quarantine); 2-after the 15 weeks of differential diet exposure; 3-after 5 weeks and 5.5 weeks of tumor growth; and 4-after 24 hours of treatment, corresponding to 6 weeks of tumor growth. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
