Summary of Study ST001721
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001103. The data can be accessed directly via it's Project DOI: 10.21228/M84988 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001721 |
Study Title | Detecting sex-related changes to the metabolome of a critically endangered freshwater crayfish during the mating season |
Study Type | LC-MS analysis of crustacean haemolymph |
Study Summary | Captive breeding is a vital tool in the conservation of highly endangered species, as it is for the Margaret River hairy marron, Cherax tenuimanus, from the south west of Australia. A close relative, Cherax cainii, has almost completely displaced C. tenuimanus in the wild and is a successful aquaculture species, whereas C. tenuimanus has performed poorly in captivity. We used untargeted liquid chromatography-mass spectrometry to obtain metabolomic profiles of female and male C. tenuimanus held in controlled aquarium conditions during their reproductive period. Using repeated haemolymph sampling we tracked the metabolomic profiles of animals just prior to and for a period of up to 34 days after pairing with a similar sized potential mate. We identified 54 reproducible annotated metabolites including amino acids, fatty acids, biogenic amines, purine and pyrimidine metabolites and excretion metabolites. Hierarchical clustering analysis distinguished five metabolite clusters. Principal component-canonical variate analysis clearly distinguished females from males, both unpaired and paired; similar trends in profile changes in both sexes after pairing; and a striking shift in males upon pairing. We discuss three main patterns of metabolomic responses: differentiation between sexes; reactive responses to the disturbance of pairing; and convergent response to the disturbance of pairing for males. Females generally had higher concentrations of metabolites involved in metabolic rate, mobilisation of energy stores and stress. Responses to the disturbance of pairing were also related to elevated stress. Females were mobilising lipid stores to deposit yolk, whereas males had a rapid and strong response to pairing, with shifts in metabolites associated with gonad development and communication, indicating males could complete reproductive readiness only once paired with a female. The metabolomic profiles support a previously proposed potential mechanism for displacement of C. tenuimanus by C. cainii in the wild and identify several biomarkers for testing hypotheses regarding reproductive success using targeted metabolomics. |
Institute | Edith Cowan University |
Department | School of Science |
Last Name | Lette |
First Name | Emily |
Address | 270 Joondalup Drive, Joondalup, WA, 6027, Australia |
e.lette@ecu.edu.au | |
Phone | +61 8 6304 5513 |
Submit Date | 2021-02-25 |
Total Subjects | 10 |
Num Males | 5 |
Num Females | 5 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2021-03-17 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
Collection ID: | CO001791 |
Collection Summary: | All haemolymph collections for all animals occurred at the same time of day and the animals were handled in the same order on each of the four collection dates. Haemolymph (200mL) was extracted from the ventral sinus of each crayfish using a 21G needle and 1mL syringe inserted into the soft tissue at the base of the 5th pereopod. Haemolymph was added to 2mL Eppendorf tubes preloaded with 600µL LC-MS grade acetonitrile (Optima, Thermo Fisher Scientific, AUS) containing deuterated internal standards (d8- valine, d9 –trimethylamine-N-oxide (TMAO) , d3-leucine, d6-trans-cinnamic acid, d5-tryptophan, 1g/mL) from Cambridge Isotope Laboratories (Cambridge, MA, USA) and stored on ice. Samples were mixed at 1400rpm (Thermal Mixer, Thermo Fisher Scientific, AUS) for 60 seconds at 4°C, then centrifuged (Heraeus Megafuge 8R, Thermo Fisher Scientific, AUS) for 20 minutes (1800 g) at 4°C. After centrifuging, 100µL of supernatant was aliquoted into five separate vials. The samples were then dried using a SpeedVac centrifugal vacuum concentrator (Thermo Fisher Scientific, AUS). The dried samples were stored at -80°C for subsequent metabolomics analysis. |
Collection Protocol Filename: | EmilyLette_20210225_014138_PR_CO_Methods__Lette_marron.pdf |
Sample Type: | Hemolymph |
Collection Method: | aspiration |
Collection Location: | ventral sinus accessed from soft tissue at base of 5th pereopod |
Storage Conditions: | -80℃ |
Collection Vials: | 2mL Eppendorf tubes |
Storage Vials: | 2mL Eppendorf tubes |