Summary of Study ST001918
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001209. The data can be accessed directly via it's Project DOI: 10.21228/M8F70B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001918 |
| Study Title | Metabolome-wide association study of occupational exposure to benzene |
| Study Summary | Benzene is a recognized hematotoxin and leukemogen; however, its mechanism of action in humans remain unclear. To provide insight into the processes underlying benzene hematotoxicity, we performed high-resolution metabolomic (HRM) profiling of plasma collected from a cross-sectional study of 33 healthy workers exposed to benzene (median 8-hr time-weighted average exposure; 20 ppma), and 25 unexposed controls in Shanghai, China. Metabolic features associated with benzene were identified using a metabolome-wide association study (MWAS) that tested for the relationship between feature intensity and benzene exposure. MWAS identified 478 mass spectral features associated with benzene exposure at FDR<20%. Comparison to a list of 13 known benzene metabolites and metabolites predicted using a multi-component biotransformation algorithm showed five metabolites were detected, which included the known metabolites phenol and benzene diolepoxide. Metabolic pathway enrichment identified 41 pathways associated with benzene exposure, with altered pathways including carnitine shuttle, fatty acid metabolism, sulfur amino acid metabolism, glycolysis, gluconeogenesis, and branched chain amino acid metabolism. These results suggest disruption to fatty acid uptake, energy metabolism and increased oxidative stress, and point towards pathways related to mitochondrial dysfunction, which has previously been linked to benzene exposure in animal models and human studies. Taken together, these results suggest benzene exposure is associated with disruption of mitochondrial pathways, and provide promising, systems biology biomarkers for risk assessment of benzene-induced hematotoxicity in humans. |
| Institute | Icahn School of Medicine at Mount Sinai |
| Department | Environmental Medicine and Public Health |
| Laboratory | High Resolution Exposomics |
| Last Name | Walker |
| First Name | Douglas |
| Address | Atran Building RM AB3-39, 1428 Madison Ave, New York, NY, 10029, USA |
| douglas.walker@mssm.edu | |
| Phone | 1-212-241-4392 |
| Submit Date | 2021-08-26 |
| Num Groups | 3 |
| Total Subjects | 58 |
| Num Males | 28 |
| Num Females | 30 |
| Publications | N Rothman, R Vermeulen, L Zhang, W Hu, S Yin, SM Rappaport, MT Smith, DP Jones, M Rahman, Qing Lan, DI Walker. (2021). Metabolome-wide association study of occupational exposure to benzene. Carcinogenesis. In Review |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-10-02 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001989 |
| Collection Summary: | Identification of factories and worker enrollment are described in detail elsewhere. [14] Study subjects were enrolled in the Fall of 1992 in Shanghai, China and evaluated at the Shanghai Hygiene and Anti-Epidemic Institute. The study protocol was explained to all potential participants, and informed consent was obtained using Institutional Review Board-approved procedures. Data collected included age, gender, tobacco use, alcohol consumption, medical history, and an occupational work history. Prior to the clinical visit, study subjects were asked to refrain from eating solid foods after dinner the night before and the morning of the clinical phase of the study. The following morning, a 27 mL sample of blood was obtained by venous phlebotomy. Plasma samples were then frozen at -80°C, shipped to the NCI on dry ice, and stored continuously at -80°C until analysis. |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
| Additives: | EDTA |
