Summary of Study ST002346
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001506. The data can be accessed directly via it's Project DOI: 10.21228/M81D8C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002346 |
| Study Title | Lipidomics study of the cells defective in peroxisome division |
| Study Summary | Proliferation of peroxisomes is accompanied by the growth and division of pre-existing peroxisomes. Pex11β induces the elongation of peroxisome membrane and then dynamin-like GTPase, DLP1, elicits the peroxisomal division. Nucleoside diphosphate kinase 3 (NME3) generates GTP for the DLP1 activity. Deficiencies of either of the factors induce abnormal morphology of peroxisomes. In this study, we assessed the phospholipid compositions in cells lacking each of the different division factors. In the fibroblasts from the patients deficient in DLP1, NME3, or Pex11β, docosahexaenoic acid (DHA, C22:6)-containing phospholipids were found to be decreased. Conversely, the levels of several fatty acids such as arachidonic acid (AA, C20:4) and oleic acid (C18:1) were elevated. Mouse embryonic fibroblasts from Drp1- and Pex11β-knockout mice also showed a decrease in the levels of phospholipids containing DHA and AA. |
| Institute | Kyushu university |
| Last Name | Abe |
| First Name | Yuichi |
| Address | 3-1-1 Maidashi, Fukuoka 812-8582, Japan. |
| y-abe@bio.sojo-u.ac.jp | |
| Phone | +81926426341 |
| Submit Date | 2022-10-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-11-28 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002428 |
| Collection Summary: | Fibroblasts and MEFs were cultured under 5% CO2 at 37°C in Dulbecco's modified medium (DMEM; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum. For the extraction of phospholipids, fibroblasts were seeded in 6-well plate and cultured overnight, followed by replacement of fresh culture medium. Cells were harvested at 48 hours after the medium change as described below. |
| Sample Type: | Cultured fibroblasts |
