Summary of Study ST002720
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001307. The data can be accessed directly via it's Project DOI: 10.21228/M8S124 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002720 |
| Study Title | Metabolic characterization of the polar endometabolome of Triple-Negative Breast Cancer parental and cDDP-resistant cells |
| Study Type | NMR-based metabolomics |
| Study Summary | Platinum (Pt(II)) drugs, e.g. cisplatin (cDDP), are some of the most used chemotherapeutic agents, yet tumor acquired resistance and high toxicity are still current drawbacks. Metabolomics can measure the metabolic response of drug-exposed cells, unveiling insight into drug mechanisms and metabolic markers of drug efficacy, toxicity and resistance. The present 1H NMR metabolomics study aims to describe the polar endometabolome of both MDA-MB-231 parental and cDDP-resistant cells (MDA-MB-231\R), which are representative of Triple-Negative Breast Cancer, aiding the current knowledge about the resistant cells metabolism rewiring and disclosing metabolic hotspots as possible targets to counteract the therapy resistance. |
| Institute | University of Aveiro |
| Department | Department of Chemistry and CICECO-Aveiro Institute of Materials |
| Laboratory | Metabolomics from Ana M. Gil |
| Last Name | Carneiro |
| First Name | Tatiana João |
| Address | Campus Universitário de Santiago, Aveiro, Aveiro, 3810-193, Portugal |
| tatiana.joao@ua.pt | |
| Phone | +351926369478 |
| Submit Date | 2023-05-30 |
| Num Groups | 6 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2024-01-01 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002819 |
| Collection Summary: | MDA-MB-231 parental and resistant (MDA-MB-231/R) cells were seeded at a density of 3 × 10^4 cells/cm2 onto 13.55 cm diameter Petri dishes, cultured in a humidified atmosphere of 5% CO2 at 37 ◦C and allowed to adhere for 24 h. After this, cells were incubated and collected at 0, 24 and 48 h, with basis on the population (25.5 ± 0.9 h and 30.6 ± 1.1 h for MDA-MB-231 and MDA-MB-231/R cells, respectively). At each time-point, cells were harvested using a 0.25% (v/v) trypsin-EDTA solution, washed twice with PBS and centrifuged (300 g, 5 min, 20 ◦C). The cell pellet was directly stored at − 80 ◦C until analysis. Three independent experiments with triplicates were performed for each cell type and time-point. |
| Sample Type: | Breast cancer cells |
| Collection Duration: | Between 2 and 5 minutes |
| Storage Conditions: | -80℃ |
