Summary of Study ST002723

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001689. The data can be accessed directly via it's Project DOI: 10.21228/M8CT6V This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002723
Study SummaryThe goal of this project is to elucidate interactions among the gut microbiome, anti-inflammatory food metabolomic signatures, and human inflammation phenotypes. Inflammation plays both direct and indirect roles in the development of type 2 diabetes (T2D), atherogenic cardiovascular diseases, and other causes of morbidity and mortality. In preliminary USDA-NIFA funded studies, we found that individuals of distinct low and high inflammation phenotypes have distinct metabolomic signatures in their blood. Anthocyanins and fiber of bioactive components of foods that have been shown to lower inflammation. However, there is tremendous inter-individual variability in bioavailability of anthocyanins and production of phenolic and aromatic metabolites in the colon that depends, at least in part, on digestive metabolism by microorganisms (the microbiota) in the gut. Fiber which acts as a prebiotic to enrich favorable gut microbes and as a fermentation substrate to produce favorable or unfavorable metabolites according to the unique makeup of the gut microbiota. However, little is known about the complex interactions among the gut microbiome, anti-inflammatory food metabolomic signatures, and human inflammation phenotypes. We propose a of human mechanistic clinical trials and mice humanized with fecal microbiome transplants to disentangle these complex interactions. To determine the metabolomic signatures anti-inflammatory foods and key bioactive components and determine associations with constituents of the gut microbiome (Aim 1A), we will measure in a human cohort the makeup of the gut microbiome and metabolomic changes induced by acute (3 d) ingestion of 1) chokeberry and chokeberry anthocyanins (n=75), and 2) lentils and lentil fiber (n=75). To determine whether these foods are related to the metabolomic signatures of low versus high inflammation phenotypes (Aim 1B), we will compare the metabolites and associated metabolic pathways of chokeberry, chokeberry anthocyanins, lentils, and lentil fiber to those associated with low and high inflammation phenotypes. To determine the impact of inter-individual variability of the gut microbiome on metabolomic signatures (Aim 2A), we will humanize mice with a diverse collection of human gut microbiomes and determine whether the makeup of the microbiome predicts features (metabolites) of chokeberry/anthocyanin, lentils/fiber metabolomic signatures. Findings from these experiments directly address the PAR-18-727 program area priority of “identification and validation of food and nutrient specific metabolic signatures that correlate with nutrient quality and efficacy and provide insights to develop synergistic food prebiotic based therapies to convert humans from high to low inflammation phenotypes to reduce disease risk and severity.
Montana State University
Last NamePeach
First NameJesse
AddressPO Box 173400, Bozeman, MT 59717
Submit Date2023-05-28
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2023-06-26
Release Version1
Jesse Peach Jesse Peach application/zip

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Collection ID:CO002822
Collection Summary:Two human stool donors were selected based on their inflammation profile which occurred prior to gut microbial community profiling. Female germ- free (GF) C57BL/6J mice, originally purchased from the Jackson Laboratory (Bar Harbor, ME) were housed and bred at the American Association for the Accreditation of Laboratory Animal Care-accredited Animal Resource Center at Montana State University. Mice were held in individually ventilated cages with sterile bedding before and after fecal transplantation from selected human stool donors. Two female mice received an inoculation with fecal material from a human donor categorized as having low or high systemic inflammation based on serum levels of six proinflammatory cytokines. Human donor stool slurry aliquots were administered to GF mice through oral gavage. Sexually mature male GF C57BL/6J mice were added to each cage approximately one week after transplantation. Male mice removed prior to birth of pups. Pups from the inoculated dams were co-housed by sex (3 – 5 mice/cage) with different microbial inoculations placed in separate isolators. Mice from each microbial inoculation were assigned to one of two juice groups: Aronia (ARO LO , = 3, ARO HI , n=5), or a sugar-matched juice (CON LO ,n=3, CON HI , n = 3). Weekly measurements of body weight and food and fluid intake were recorded. Blood samples were collected at the same interval into serum separating tubes. Whole blood was allowed to clot for 15 minutes before centrifugation at 1200 RPM for 15 minutes with resulting serum aliquoted and stored at -80ºC until analysis. After T8 sample collection, mice were euthanized via rapid CO 2 asphyxiation.
Sample Type:Blood (serum)