Summary of Study ST002891
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001804. The data can be accessed directly via it's Project DOI: 10.21228/M8HX5R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002891 |
| Study Title | Glutamine metabolism improves left ventricular function but not macrophage-mediated inflammation following myocardial infarction |
| Study Type | untargeted metabolomics analysis |
| Study Summary | Glutamine is a critical amino acid that serves as an energy source, building block, and signaling molecule for both the heart tissue and the immune system. However, the role of glutamine metabolism in regulating cardiac remodeling following myocardial infarction (MI) is unknown. In this study, we show that glutamine metabolism is altered both in the remote (contractile) area and in infiltrating macrophages in the infarct area after MI in adult male mice by permanent left anterior descending artery occlusion. Using untargeted metabolomics in extracted LV macrophages, we found that metabolites related to glutamine metabolism were differentially altered at days 1, 3, and 7 after MI. Glutamine metabolism in live cells was found to be increased after MI relative to no MI controls. Gene expression in the remote area of the heart indicated a loss of glutamine metabolism. Glutamine administration improved LV function at days 1, 3, and 7, which was associated with improved contractile and metabolic gene expression. |
| Institute | Vanderbilt University |
| Department | Chemistry |
| Laboratory | Center for Innovative Technology |
| Last Name | Codreanu |
| First Name | Simona |
| Address | 1234 STEVENSON CENTER LANE, NASHVILLE, TN, 37235, USA |
| SIMONA.CODREANU@VANDERBILT.EDU | |
| Phone | 6158758422 |
| Submit Date | 2023-09-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-04-02 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002997 |
| Collection Summary: | MI was induced in adult (15-20 week old) male C57BL/6J mice as previously described.2,4,6 Mice were anesthetized (2% isoflurane) and laid supine on a heated stage (37°C), intubated and connected to a mouse ventilator (Harvard Apparatus; 300µL stroke volume, 300 breaths/min). The chest was incised to expose the ribs, and the heart exposed between the 3rd and 4th ribs. The pericardial layer was gently removed, and the left coronary artery was ligated ~1mm below the left atrium using 8-0 suture. Ischemia was confirmed by blanching of the LV. For glutamine administration, mice were injected intraperitoneally with glutamine (0.75-1g/kg) or saline vehicle beginning at either day 1 post-MI (for day 3 time-point) or beginning at day 3 (for day 7 time-point). A separate cohort of mice was injected 2 hr after MI and studied 24 hr later (day 1). To inhibit glutamine metabolism, mice were administered BPTES (12.5mg/kg; 10% in DMSO/corn oil), a glutaminase-1 inhibitor, beginning at either day 1 or day 3. |
| Sample Type: | Macrophages |
