Summary of Study ST003008

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001873. The data can be accessed directly via it's Project DOI: 10.21228/M8MF0Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003008
Study Title13C BCAA tracing in differentiated brown adipocyte
Study SummaryTo determine the metabolic fate and carbon flux of BCAA in mouse brown adipocytes, we used 13C labeled BCAA tracing (Leu (NLM-142-1, CIL), Ile (NLM-292-0.25, CIL) and Val (NLM-316-0.5, CIL)) followed by LC-MS analysis.
Institute
Harvard Medical School
Last NameWang
First NameDandan
Address3 Blackfan Circle
Emaildandanwang2022@gmail.com
Phone5083733714
Submit Date2023-12-13
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-02-19
Release Version1
Dandan Wang Dandan Wang
https://dx.doi.org/10.21228/M8MF0Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO003115
Collection Summary:For the metabolite extraction from adipocytes, after aspirating the tracing media, the cells were immediately incubated with 500 μL cold methanol containing 1ug/ml internal standard (D8-Phe) for 5 min on dry ice and then scrapped into the Eppendorf tubes.
Sample Type:Adipocytes
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