Summary of Study ST000560

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000411. The data can be accessed directly via it's Project DOI: 10.21228/M8XG7R This work is supported by NIH grant, U2C- DK119886.

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Study IDST000560
Study TitleMetabolomics of immunoglobulin-producing cells in IgA nephropathy
Study SummaryIgA nephropathy (IgAN), the most common primary glomerulonephritis, is characterized by deposits of IgA-containing immune complexes in the kidney glomeruli, as first described by Berger and Hinglais in 1968. IgAN is a major cause of end-stage renal disease with its associated cardio-renal morbidity and mortality. Analyses of the IgA deposits revealed that the IgA is exclusively of the IgA1 subclass and that this IgA1 is aberrantly glycosylated, deficient in galactose in some O-glycans (Gd-IgA1). Patients with IgAN have elevated serum levels of Gd-IgA1 bound by anti-glycan autoantibodies in circulating immune complexes (CIC) that are fundamental in driving disease pathology in an autoimmune process. We have recently shown that elevated serum levels of Gd-IgA1 in patients with IgAN predict disease progression. Thus, understanding the mechanisms behind Gd-IgA1 production will improve future treatment options, as there is presently no disease-specific therapy. A total of 24 cell pellets (4 replicates from 6 cell lines) were analyzed by LCMS metabolomics. Immortalized immunoglobulin-producing cell lines were generated from peripheral-blood lymphocytes from patients with IgAN and healthy controls as described in Suzuki, H., Moldoveanu, Z., Hall, S., et al. IgA1-secreting cell lines from patients with IgA nephropathy produce aberrantly glycosylated IgA1. J Clin Invest. 2008, 118, 629-639.
Institute
RTI International
LaboratoryNIH Eastern Regional Comphrehensive Metabolomics Resource Core at UNC Chapel Hill (ERCMRC)
Last NameSumner
First NameSusan
Address3040 E. Cornwallis Road, Research Triangle Park, NC 27709
Emailsusan_sumner@unc.edu
Phone704-250-5000
Submit Date2017-02-17
Num Groups2
Total Subjects24
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2018-04-10
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8XG7R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Phenotype
SA028898HC3_2HC
SA028899HC3_3HC
SA028900HC3_4HC
SA028901HC2_4HC
SA028902HC1_1HC
SA028903HC3_1HC
SA028904HC2_3HC
SA028905HC1_2HC
SA028906HC1_4HC
SA028907HC1_3HC
SA028908HC2_1HC
SA028909HC2_2HC
SA028910IgAN3_2IgAN
SA028911IgAN3_1IgAN
SA028912IgAN2_4IgAN
SA028913IgAN3_4IgAN
SA028914IgAN3_3IgAN
SA028915IgAN1_3IgAN
SA028916IgAN2_3IgAN
SA028917IgAN1_1IgAN
SA028918IgAN1_2IgAN
SA028919IgAN1_4IgAN
SA028920IgAN2_2IgAN
SA028921IgAN2_1IgAN
SA028922Pool_5Pool
SA028923Pool_4Pool
SA028924Pool_1Pool
SA028925Pool_2Pool
SA028926Pool_3Pool
Showing results 1 to 29 of 29
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