Summary of Study ST000921

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000637. The data can be accessed directly via it's Project DOI: 10.21228/M8B96C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST000921
Study Title	Karenia brevis allelopathy compromises the lipidome, membrane integrity, and photosynthetic efficiency of competitors
Study Type	Untargeted lipidomics
Study Summary	Allelopathy, or the release of compounds that inhibit competitors, is a form of interference competition that is common among bloom-forming phytoplankton. Allelopathy is hypothesized to play a role in bloom propagation and maintenance and is well established in the red tide dinoflagellate Karenia brevis. K. brevis typically suppresses competitor growth through unknown mechanisms over the course of many days. When we investigated the effects of allelopathy on the lipidomes of two competing phytoplankton, Asterionellopsis glacialis and Thalassiosira pseudonana using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS)- based metabolomics, we found that the lipidomes of both species were significantly altered, however A. glacialis maintained a more robust response whereas T. pseudonana saw significant alterations in fatty acid synthesis, cell membrane integrity, and a decrease in photosynthetic efficiency. Membrane- associated lipids were significantly suppressed for T. pseudonana exposed to allelopathy to the point of permeabilizing the cell membrane of living cells. The dominant mechanisms of K. brevis allelopathy appear to target lipid biosynthesis affecting multiple physiological pathways suggesting that exuded compounds have the ability to significantly alter competitor physiology and give K. brevis a competitive edge over sensitive species.
Institute	Georgia Institute of Technology
Department	Chemistry
Laboratory	Fernández
Last Name	Hogan
First Name	Scott
Address	901 Atlantic Drive, Atlanta, GA, 30332, USA
Email	srjhogan@gatech.edu
Phone	2156924657
Submit Date	2018-01-19
Num Groups	4
Total Subjects	51
Raw Data Available	No
Raw Data File Type(s)	raw(Waters)
Analysis Type Detail	LC-MS
Release Date	2018-08-27
Release Version	1

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Factors:

Subject type: Plankton; Subject species: Thalassiosira pseudonana;Karenia brevis (Factor headings shown in green)

mb_sample_id	local_sample_id	Class
SA054646	TpC6	Control
SA054647	TpC11	Control
SA054648	TpC4	Control
SA054649	TpC12	Control
SA054650	TpC9	Control
SA054651	TpC15	Control
SA054652	TpC3	Control
SA054653	AgC2	Control
SA054654	TpC2	Control
SA054655	TpC5	Control
SA054656	TpC10	Control
SA054657	TpC8	Control
SA054658	TpC13	Control
SA054659	TpC7	Control
SA054660	AgC3	Control
SA054661	AgC5	Control
SA054662	AgC1	Control
SA054663	AgC6	Control
SA054664	AgC11	Control
SA054665	AgC4	Control
SA054666	AgC8	Control
SA054667	AgC12	Control
SA054668	AgC7	Control
SA054669	AgC9	Control
SA054670	AgC15	Control
SA054671	AgC10	Control
SA054672	TpT4	treatment
SA054673	TpT3	treatment
SA054674	TpT6	treatment
SA054675	TpT15	treatment
SA054676	TpT11	treatment
SA054677	TpT7	treatment
SA054678	TpT8	treatment
SA054679	TpT13	treatment
SA054680	TpT10	treatment
SA054681	TpT2	treatment
SA054682	TpT9	treatment
SA054683	TpT5	treatment
SA054684	AgT1	treatment
SA054685	AgT7	treatment
SA054686	AgT10	treatment
SA054687	AgT8	treatment
SA054688	AgT15	treatment
SA054689	AgT2	treatment
SA054690	AgT9	treatment
SA054691	AgT5	treatment
SA054692	AgT3	treatment
SA054693	AgT4	treatment
SA054694	AgT6	treatment
SA054695	AgT12	treatment
SA054696	AgT11	treatment

Showing results 1 to 51 of 51

Showing results 1 to 51 of 51