Summary of Study ST001489

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001006. The data can be accessed directly via it's Project DOI: 10.21228/M8NT36 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001489
Study TitleMetabolomics by UHPLC-HRMS reveals the impact of heat stress on pathogen-elicited immunity in maize
Study SummaryStudies investigating crop resistance to biotic and abiotic stress have largely focused on plant responses to singular forms of stress and individual biochemical pathways that only partially represent stress responses. Thus, combined biotic and abiotic stress treatments and the global assessment of their elicited metabolic expression remains largely unexplored. In this study, we employed targeted and untargeted metabolomics to investigate the metabolic responses of maize (Zea mays) to both individual and combinatorial stress treatments using heat (abiotic) and Cochliobolus heterostrophus infection (biotic) experiments. Ultra-high-performance liquid chromatography-high-resolution mass spectrometry revealed significant metabolic responses to C. heterostrophus infection and heat stress, and comparative analyses between these individual forms of stress demonstrated differential elicitation between the two global metabolomes. In combinatorial experiments, treatment with heat stress prior to fungal inoculation negatively impacted maize disease resistance against C. heterostrophus, and distinct metabolome separation between combinatorial stressed plants and the non-heat stressed infected controls was observed. Targeted analysis revealed inducible primary and secondary metabolite responses to biotic/abiotic stress, and combinatorial experiments indicated that deficiency in the hydroxycinnamic acid, p-coumaric acid, may lead to the heat-induced susceptibility of maize to C. heterostrophus. Collectively, these findings demonstrate that abiotic stress can predispose crops to more severe disease symptoms, underlining the increasing need to investigate defense chemistry in plants under combinatorial stress.
Institute
Agricultural Research Service, United States Department of Agriculture
DepartmentCenter of Medical, Agricultural, and Veterinary Entomology
LaboratoryChemistry Research Unit
Last NameChristensen
First NameShawn
Address1600 SW 23rd Drive Gainesville, FL 32608
Emailshawn.christensen@usda.gov
Phone3523745739
Submit Date2020-08-03
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-08-03
Release Version1
Shawn Christensen Shawn Christensen
https://dx.doi.org/10.21228/M8NT36
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type Age Growth Temperature
SA1254158_Control 14 day 28
SA1254169_Control 14 day 28
SA12541712_Control 14 day 28
SA1254187_Control 14 day 28
SA12541910_Control 14 day 28
SA12542011_Control 14 day 28
SA12542131_Control 14 day 38 returned to 28
SA12542232_Control 14 day 38 returned to 28
SA12542333_Control 14 day 38 returned to 28
SA12542434_Control 14 day 38 returned to 28
SA12542556_Control 14 day 38 returned to 38
SA12542657_Control 14 day 38 returned to 38
SA12542758_Control 14 day 38 returned to 38
SA12542855_Control 14 day 38 returned to 38
SA12542959_Control 14 day 38 returned to 38
SA1254304_SLB inoculation 14 day 28
SA1254315_SLB inoculation 14 day 28
SA1254322_SLB inoculation 14 day 28
SA1254331_SLB inoculation 14 day 28
SA1254346_SLB inoculation 14 day 28
SA1254353_SLB inoculation 14 day 28
SA12543629_SLB inoculation 14 day 38 returned to 28
SA12543728_SLB inoculation 14 day 38 returned to 28
SA12543827_SLB inoculation 14 day 38 returned to 28
SA12543926_SLB inoculation 14 day 38 returned to 28
SA12544025_SLB inoculation 14 day 38 returned to 28
SA12544130_SLB inoculation 14 day 38 returned to 28
SA12544249_SLB inoculation 14 day 38 returned to 38
SA12544351_SLB inoculation 14 day 38 returned to 38
SA12544452_SLB inoculation 14 day 38 returned to 38
SA12544553_SLB inoculation 14 day 38 returned to 38
SA12544654_SLB inoculation 14 day 38 returned to 38
SA12544750_SLB inoculation 14 day 38 returned to 38
Showing results 1 to 33 of 33
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