Summary of Study ST002062

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001283. The data can be accessed directly via it's Project DOI: 10.21228/M8VX1B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples  |  Perform analysis on untargeted data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002062
Study TitleEndophytic bacteria are key players in the modulation of the secondary metabolome of Lithospermum officinale L.
Study SummaryEndophytic bacteria influence plant growth and development and therefore are an attractive resource for applications in agriculture. However, little is known about the impact of these microorganisms on secondary metabolite (SM) production by medicinal plants. Here we assessed, for the first time, the effects of root endophytic bacteria on the modulation of SMs in the medicinal plant Lithospermum officinale (Boraginaceae family), with a focus on the naphthoquinones alkannin/shikonin (A/S). The study was conducted using a newly developed in vitro system as well as in the greenhouse. Targeted and non-targeted metabolomics approaches were used and supported by expression analysis of the gene PGT, encoding a key enzyme in the A/S biosynthesis pathway. Three bacterial strains, Chitinophaga sp. R-73072, Xanthomonas sp. R-73098 and Pseudomonas sp. R-71838 induced a significant increase of diverse SMs, including A/S, in L. officinale in both systems, demonstrating the strength of our approach for screening A/S derivative-inducing bacteria. Our results highlight the impact of root-endophytic bacteria on secondary metabolism in plants and indicate that production of A/S derivatives in planta likely involves cross-modulation of different metabolic pathways that can be manipulated by bacterial endophytes.
Institute
Aristotle University of Thessaloniki
Last NameRodic
First NameNebojsa
AddressStepe Stepanovica 5, Conoplja, Serbia
Emailnebojsa.rodic@hotmail.com
Phone+381648766400
Submit Date2021-08-11
Num Groups7
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-01-31
Release Version1
Nebojsa Rodic Nebojsa Rodic
https://dx.doi.org/10.21228/M8VX1B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Plant; Subject species: Lithospermum officinale (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA19403371875_3Brevibacterium sp. R-71875
SA19403471875_1Brevibacterium sp. R-71875
SA19403571875_4Brevibacterium sp. R-71875
SA19403671875_5Brevibacterium sp. R-71875
SA19403771875_6Brevibacterium sp. R-71875
SA19403871875_2Brevibacterium sp. R-71875
SA19403972269_2Chitinophaga sp. R-72269
SA19404072269_6Chitinophaga sp. R-72269
SA19404172269_4Chitinophaga sp. R-72269
SA19404272269_3Chitinophaga sp. R-72269
SA19404372269_1Chitinophaga sp. R-72269
SA19404472269_5Chitinophaga sp. R-72269
SA19404573072_1Chitinophaga sp. R-73072
SA19404673072_6Chitinophaga sp. R-73072
SA19404773072_4Chitinophaga sp. R-73072
SA19404873072_3Chitinophaga sp. R-73072
SA19404973072_2Chitinophaga sp. R-73072
SA19405073072_5Chitinophaga sp. R-73072
SA194051Cminus_5Control
SA194052Cminus_2Control
SA194053Cminus_3Control
SA194054Cminus_4Control
SA194055Cminus_6Control
SA194056Cminus_1Control
SA19405771838_2Pseudomonas sp. R-71838
SA19405871838_3Pseudomonas sp. R-71838
SA19405971838_6Pseudomonas sp. R-71838
SA19406071838_4Pseudomonas sp. R-71838
SA19406171838_1Pseudomonas sp. R-71838
SA19406271838_5Pseudomonas sp. R-71838
SA194063QC_6_BACTERIA_LE_24_03QC
SA194064QC_6_BACTERIA_LE_24_01QC
SA194065QC_6_BACTERIA_LE_24_02QC
SA19406672433_5Rhizobium sp. R-72433
SA19406772433_4Rhizobium sp. R-72433
SA19406872433_3Rhizobium sp. R-72433
SA19406972433_2Rhizobium sp. R-72433
SA19407072433_1Rhizobium sp. R-72433
SA19407172433_6Rhizobium sp. R-72433
SA19407273098_1Xanthomonas sp. R-73098
SA19407373098_5Xanthomonas sp. R-73098
SA19407473098_4Xanthomonas sp. R-73098
SA19407573098_3Xanthomonas sp. R-73098
SA19407673098_2Xanthomonas sp. R-73098
SA19407773098_6Xanthomonas sp. R-73098
Showing results 1 to 45 of 45
  logo