Summary of Study ST002227

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001418. The data can be accessed directly via it's Project DOI: 10.21228/M8F409 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002227
Study TitleAssessing the contribution of branched-chain amino acid (BCAA)-derived nitrogen to amino acid biosynthesis in renal cell lines HK2 and 786-O.
Study SummaryThe objective of this experiment is to test the contribution of branched-chain amino acids (BCAA)-derived nitrogen to the generation of de novo amino acids in renal cells through transamination reactions. To test this hypothesis, we incubated human renal epithelial cell line HK2 and ccRCC cell lines 786-O, 786-M1A and 786-M2A with 15N-leucine and 15N-isoleucine in Plasmax media for 27h. Data were generated from 5 independent cultures. This is Part 2 of the study and the experimental number is MS48.
Institute
CECAD Research Center
Last NameYang
First NameMing
AddressJoseph-Stelzmann-Straße 26, Köln, Koeln, 50931, Germany
Emailming.yang@uni-koeln.de
Phone4922147884306
Submit Date2022-07-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-08-03
Release Version1
Ming Yang Ming Yang
https://dx.doi.org/10.21228/M8F409
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line Treatment
SA212335MS48-13786-M1A 15N-leucine+15N-isoleucine
SA212336MS48-14786-M1A 15N-leucine+15N-isoleucine
SA212337MS48-15786-M1A 15N-leucine+15N-isoleucine
SA212338MS48-12786-M1A 15N-leucine+15N-isoleucine
SA212339MS49-11786-M1A 15N-leucine+15N-isoleucine
SA212340MS48-18786-M2A 15N-leucine+15N-isoleucine
SA212341MS48-19786-M2A 15N-leucine+15N-isoleucine
SA212342MS48-16786-M2A 15N-leucine+15N-isoleucine
SA212343MS48-20786-M2A 15N-leucine+15N-isoleucine
SA212344MS48-17786-M2A 15N-leucine+15N-isoleucine
SA212345MS48-07786-O 15N-leucine+15N-isoleucine
SA212346MS48-06786-O 15N-leucine+15N-isoleucine
SA212347MS48-08786-O 15N-leucine+15N-isoleucine
SA212348MS48-09786-O 15N-leucine+15N-isoleucine
SA212349MS48-10786-O 15N-leucine+15N-isoleucine
SA212350MS48-02HK2 15N-leucine+15N-isoleucine
SA212351MS48-04HK2 15N-leucine+15N-isoleucine
SA212352MS48-01HK2 15N-leucine+15N-isoleucine
SA212353MS48-05HK2 15N-leucine+15N-isoleucine
SA212354MS48-03HK2 15N-leucine+15N-isoleucine
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