Summary of Study ST002342

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001504. The data can be accessed directly via it's Project DOI: 10.21228/M88X2F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002342
Study Title	The gut microbiome has sexually dimorphic effects on bone tissue energy metabolism and multiscale bone quality in C57BL/6J mice
Study Summary	Male and female germ-free and conventional were euthanized at 20 weeks of age, and various tissues were obtained to assess differences in bone properties associated with microbiome status and sex. For metabolomic analyses, the humeri was obtained, bone marrow was flushed using PBS, and subchondral bone metabolites were extracted using a methanol:acetone precipitation approach. Next, samples were injected, ionized, and data was acquired using LC-MS. Data was processed and converted using MSConvert and XCMS. Following this step, metabolic phenotypes of mice that differ by microbiome status and sex were investigated using MetaboAnalyst. Differences in metabolism were related to lipid, energy, and amino acid metabolism. Specifically, females, both conventional and germ-free, had dysregulated lipid metabolism, whereas, males, both conventional and germ-free had dysregulated amino acid metabolism. Other differences detected between male and female mice that differ by germ-free status corresponded to differences in bone strength, bone marrow adiposity, and osteocyte density.
Institute	Montana State University
Department	Mechanical & Industrial Engineering, Chemistry & Biochemistry
Last Name	Welhaven
First Name	Hope
Address	Culbertson Hall, 100
Email	hwelhaven@gmail.com
Phone	4066961526
Submit Date	2022-10-19
Num Groups	4
Total Subjects	33
Num Males	17
Num Females	16
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2022-11-28
Release Version	1

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Sex	Microbiome Status
SA235201	H33	Female	Control
SA235202	H34	Female	Control
SA235203	H35	Female	Control
SA235204	H32	Female	Control
SA235205	H27	Female	Control
SA235206	H30	Female	Control
SA235207	H29	Female	Control
SA235208	H28	Female	Control
SA235209	H36	Female	Control
SA235210	H31	Female	Control
SA235211	H18	Female	Germ-Free
SA235212	H20	Female	Germ-Free
SA235213	H3	Female	Germ-Free
SA235214	H1	Female	Germ-Free
SA235215	H19	Female	Germ-Free
SA235216	H2	Female	Germ-Free
SA235217	H5	Male	Control
SA235218	H6	Male	Control
SA235219	H4	Male	Control
SA235220	H13	Male	Control
SA235221	H7	Male	Control
SA235222	H8	Male	Control
SA235223	H11	Male	Control
SA235224	H12	Male	Control
SA235225	H9	Male	Control
SA235226	H10	Male	Control
SA235227	H17	Male	Germ-Free
SA235228	H15	Male	Germ-Free
SA235229	H14	Male	Germ-Free
SA235230	H16	Male	Germ-Free
SA235231	H21	Male	Germ-Free
SA235232	H22	Male	Germ-Free
SA235233	H23	Male	Germ-Free

Showing results 1 to 33 of 33

Showing results 1 to 33 of 33