Summary of Study ST002353

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002353
Study Title	Biomolecular condensates create phospholipid-enriched microenvironments (Part 3)
Study Type	Metabolomes of in vitro synthesized condensates
Study Summary	Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1.
Institute	Cornell University
Department	Department of Pharmacology
Laboratory	Dr. Samie Jaffrey
Last Name	Dumelie
First Name	Jason
Address	1300 York Ave, LC-524, New York City, NY
Email	jdumes98@gmail.com
Phone	6465690174
Submit Date	2022-11-16
Raw Data Available	Yes
Raw Data File Type(s)	mzdata.xml
Analysis Type Detail	LC-MS
Release Date	2023-03-01
Release Version	2

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Factors:

Subject type: Synthetic sample; Subject species: - (Factor headings shown in green)

mb_sample_id	local_sample_id	metabolite_source	protein	RNA	fraction
SA249779	MED1 Library 100 nM Aqueous Sample 3	100 nM library	MED1	150 nM	aqueous
SA249780	MED1 Library 100 nM Aqueous Sample 2	100 nM library	MED1	150 nM	aqueous
SA249781	MED1 Library 100 nM Aqueous Sample 1	100 nM library	MED1	150 nM	aqueous
SA249782	MED1 Library 100 nM Condensate Sample 1	100 nM library	MED1	150 nM	condensate
SA249783	MED1 Library 100 nM Condensate Sample 3	100 nM library	MED1	150 nM	condensate
SA249784	MED1 Library 100 nM Condensate Sample 2	100 nM library	MED1	150 nM	condensate
SA249785	MED1 Library 100 nM Input Sample 3	100 nM library	MED1	150 nM	input
SA249786	MED1 Library 100 nM Input Sample 1	100 nM library	MED1	150 nM	input
SA249787	MED1 Library 100 nM Input Sample 2	100 nM library	MED1	150 nM	input
SA249770	MED1 Library 10 uM Aqueous Sample 3	10 uM library	MED1	150 nM	aqueous
SA249771	MED1 Library 10 uM Aqueous Sample 2	10 uM library	MED1	150 nM	aqueous
SA249772	MED1 Library 10 uM Aqueous Sample 1	10 uM library	MED1	150 nM	aqueous
SA249773	MED1 Library 10 uM Condensate Sample 2	10 uM library	MED1	150 nM	condensate
SA249774	MED1 Library 10 uM Condensate Sample 1	10 uM library	MED1	150 nM	condensate
SA249775	MED1 Library 10 uM Condensate Sample 3	10 uM library	MED1	150 nM	condensate
SA249776	MED1 Library 10 uM Input Sample 3	10 uM library	MED1	150 nM	input
SA249777	MED1 Library 10 uM Input Sample 2	10 uM library	MED1	150 nM	input
SA249778	MED1 Library 10 uM Input Sample 1	10 uM library	MED1	150 nM	input
SA249761	MED1 Library 1 uM Aqueous Sample 2	1 uM library	MED1	150 nM	aqueous
SA249762	MED1 Library 1 uM Aqueous Sample 3	1 uM library	MED1	150 nM	aqueous
SA249763	MED1 Library 1 uM Aqueous Sample 1	1 uM library	MED1	150 nM	aqueous
SA249764	MED1 Library 1 uM Condensate Sample 1	1 uM library	MED1	150 nM	condensate
SA249765	MED1 Library 1 uM Condensate Sample 3	1 uM library	MED1	150 nM	condensate
SA249766	MED1 Library 1 uM Condensate Sample 2	1 uM library	MED1	150 nM	condensate
SA249767	MED1 Library 1 uM Input Sample 2	1 uM library	MED1	150 nM	input
SA249768	MED1 Library 1 uM Input Sample 1	1 uM library	MED1	150 nM	input
SA249769	MED1 Library 1 uM Input Sample 3	1 uM library	MED1	150 nM	input

Showing results 1 to 27 of 27

Showing results 1 to 27 of 27