Summary of Study ST002433

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001566. The data can be accessed directly via it's Project DOI: 10.21228/M88M5G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002433
Study TitleElucidating dynamic anaerobe metabolism with HRMAS 13C NMR and genome-scale modeling
Study SummaryAnaerobic microbial metabolism drives critical functions within global ecosystems, host-microbiota interactions, and industrial applications, yet remains ill-defined. Here we advance a versatile approach to elaborate cellular metabolism in obligate anaerobes using the pathogen Clostridioides difficile, an amino acid and carbohydrate-fermenting Clostridia. High-Resolution Magic Angle Spinning (HRMAS) Nuclear Magnetic Resonance (NMR) spectroscopy of C. difficile, grown with fermentable 13C substrates, informed dynamic flux balance analysis (dFBA) of the pathogen’s genome-scale metabolism. Analyses identified dynamic recruitment of oxidative and supporting reductive pathways, with integration of high-flux amino acid and glycolytic metabolism at alanine’s biosynthesis to support efficient energy generation, nitrogen handling, and biomass generation. Model predictions informed an approach leveraging the sensitivity of 13C NMR spectroscopy to simultaneously track cellular carbon and nitrogen flow from [U-13C]glucose and [15N]leucine, confirming the formation of [13C,15N]alanine. Findings identify metabolic strategies used by C. difficile to support its rapid colonization and expansion in gut ecosystems.
Institute
Brigham and Women's Hospital
DepartmentPathology
LaboratoryBry Lab, Massachusetts Host-Microbiome Center; Cheng Lab, Massachusetts General Hospital
Last NamePavao
First NameAidan
Address221 Longwood Ave, EBRC-411, Boston, MA, 02115, USA
Emailapavao2@bwh.harvard.edu
Phone617-525-7184
Submit Date2023-01-05
Num Groups8
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2023-01-20
Release Version1
Aidan Pavao Aidan Pavao
https://dx.doi.org/10.21228/M88M5G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Bacteria; Subject species: Clostridioides difficile (Factor headings shown in green)

mb_sample_id local_sample_id Condition
SA243357Data8_13CGlc213C-Glucose
SA243358Data9_13CGlc313C-Glucose
SA243359Data12_13CGlc_endpt313C-Glucose
SA243360Data7_13CGlc113C-Glucose
SA243361Data10_13CGlc_endpt113C-Glucose
SA243362Data11_13CGlc_endpt213C-Glucose
SA243363Data19_13CGlc_standards13C-Glucose, 13C-Acetate, 13C-Alanine, 13C-Ethanol, 13C-Butyrate
SA243364Data15_13CGlc_15NLeu_endpt313C-Glucose, 15N-Leucine
SA243365Data16_13CGlc_15NLeu_stack13C-Glucose, 15N-Leucine
SA243366Data13_13CGlc_15NLeu_endpt113C-Glucose, 15N-Leucine
SA243367Data14_13CGlc_15NLeu_endpt213C-Glucose, 15N-Leucine
SA243368Data5_13CLeu213C-Leucine
SA243369Data4_13CLeu113C-Leucine
SA243370Data6_13CLeu313C-Leucine
SA243371Data2_13CPro213C-Proline
SA243372Data3_13CPro313C-Proline
SA243373Data1_13CPro113C-Proline
SA243374Data20_2D13C-Proline, 13C-Leucine, 13C-Glucose
SA243376Data18_13CPro-Se13C-Proline, no Selenium source
SA243375Data17_13CPro+Se13C-Proline, Sodium Selenite
Showing results 1 to 20 of 20
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