Summary of Study ST002565
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001653. The data can be accessed directly via it's Project DOI: 10.21228/M81Q6Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002565 |
| Study Title | Metabolomic profiling of PMM2-CDG after siRNA mediated KD of AKR1b1 - 13C6 glucose and fructose study |
| Study Summary | Abnormal polyol metabolism has been predominantly associated with diabetes, where excess glucose is converted to sorbitol by aldose reductase (AR). Recently, abnormal polyol metabolism has also been implicated in phosphomannomutase 2-congenital disorder of glycosylation (PMM2-CDG), and epalrestat, an AR inhibitor, proposed as a potential therapy for this disorder. Given that the PMM enzyme is not closely connected to polyol metabolism, and, unlike in diabetes, PMM2-CDG does not present with hyperglycemia in blood, the increased polyol production, and the therapeutic mechanism of epalrestat in PMM2-CDG remained largely elusive. PMM2-CDG is caused by deficiency of the PMM enzyme and results in a depletion of mannose-1-P and guanosine diphosphate mannose (GDP-mannose), which is essential for glycosylation. Here, we show that apart from glycosylation abnormalities, PMM2 deficiency also leads to changes in intracellular glucose flux, which results in an increase in intracellular polyols. Ssing tracer glucose studies, we demonstrate that AR inhibition diverts glucose flux away from polyol production towards the synthesis of sugar nucleotides. |
| Institute | Mayo Clinic |
| Last Name | Radenkovic |
| First Name | Silvia |
| Address | 200 2nd Ave SW Rochester MN, USA |
| radenkovic.silvia@mayo.edu | |
| Phone | 507(77) 6-6107 |
| Submit Date | 2023-04-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-18 |
| Release Version | 1 |
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Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype | Treatment |
|---|---|---|---|
| SA257927 | SR08 | PMM2-CDG | 12C fructose + 12C glucose |
| SA257928 | SR04 | PMM2-CDG | 12C fructose + 12C glucose |
| SA257929 | SR03 | PMM2-CDG | 12C fructose + 12C glucose |
| SA257930 | SR07 | PMM2-CDG | 12C fructose + 12C glucose |
| SA257931 | SR05 | PMM2-CDG | 12C fructose+13C fructose |
| SA257932 | SR06 | PMM2-CDG | 12C fructose+13C fructose |
| SA257933 | SR02 | PMM2-CDG | 13C glu + 12C fructose |
| SA257934 | SR01 | PMM2-CDG | 13C glu + 12C fructose |
| SA257935 | SR10 | PMM2-CDG | 5.5mM 13C glucose |
| SA257936 | SR09 | PMM2-CDG | 5.5mM 13C glucose |
| SA257937 | SR11 | PMM2-CDG | 5.5mM glucose + |
| SA257938 | SR12 | PMM2-CDG | 5.5mM glucose + |
| SA257939 | SR19 | WT | 12C fructose + 12C glucose |
| SA257940 | SR16 | WT | 12C fructose + 12C glucose |
| SA257941 | SR15 | WT | 12C fructose + 12C glucose |
| SA257942 | SR20 | WT | 12C fructose + 12C glucose |
| SA257943 | SR18 | WT | 12C fructose+13C fructose |
| SA257944 | SR17 | WT | 12C fructose+13C fructose |
| SA257945 | SR13 | WT | 13C glu + 12C fructose |
| SA257946 | SR14 | WT | 13C glu + 12C fructose |
| SA257947 | SR24 | WT | 5.5mM 13C glucose |
| SA257948 | SR23 | WT | 5.5mM 13C glucose |
| SA257949 | SR22 | WT | 5.5mM glucose + |
| SA257950 | SR21 | WT | 5.5mM glucose + |
| Showing results 1 to 24 of 24 |
