Summary of Study ST002586

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001663. The data can be accessed directly via it's Project DOI: 10.21228/M8R72W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002586
Study TitleStable isotope tracer analysis (SITA) using 13C6-glucose and 13C5-glutamine measured by GC/MS in BIN-67 cells ± SMARCA4 restoration
Study SummarySMARCA4/2-loss drives the metabolic shift preferring glutamine than glucose to sustain the TCA cycle. SITA using 13C6-glucose confirmed that glucose uptake was increased in BIN-67 cells upon SMARCA4-restoration, leading to overall increased metabolic flux through glucose (lactate) and TCA cycle intermediates (citrate, fumarate, malate, aspartate) via pyruvate carboxylation.Conversely, the 13C5-glutamine SITA in BIN-67 cells revealed that SMARCA4-restoration decreased glutamine uptake and utilization via glutaminolysis to fuel the TCA cycle as indicated by reduced glutamine metabolites (glutamate, α-KG, m+5 metabolites) and TCA cycle intermediates (succinate, fumarate, malate, aspartate, m+4 metabolites)
Institute
McGill University
DepartmentBiochemistry
LaboratorySidong Huang Lab
Last NameFu
First NameZheng
AddressMcIntyre Medical Sciences Building, 3655 promenade Sir-William-Osler
Emailzheng.fu2@mail.mcgill.ca
Phone5143985446
Submit Date2023-04-25
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailGC-MS
Release Date2023-05-22
Release Version1
Zheng Fu Zheng Fu
https://dx.doi.org/10.21228/M8R72W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA25919323_Ala_C13_Glutamine_3Alanine 18h and C13 Glutamine 60min
SA25919422_Ala_C13_Glutamine_2Alanine 18h and C13 Glutamine 60min
SA25919521_Ala_C13_Glutamine_1Alanine 18h and C13 Glutamine 60min
SA25919624_Ala_C13_Glutamine_4Alanine 18h and C13 Glutamine 60min
SA25919725_Ala_GlutamineAlanine 18h and Glutamine 60min
SA2591982_C13_Glucose_2C13 Glucose 30min
SA2591991_C13_Glucose_1C13 Glucose 30min
SA2592003_C13_Glucose_3C13 Glucose 30min
SA2592014_C13_Glucose_4C13 Glucose 30min
SA25920214_C13_Glutamine_4C13 Glutamine 60min
SA25920313_C13_Glutamine_3C13 Glutamine 60min
SA25920411_C13_Glutamine_1C13 Glutamine 60min
SA25920512_C13_Glutamine_2C13 Glutamine 60min
SA2592065_GlucoseGlucose 30min
SA25920715_GlutamineGlutamine 60min
SA2592088_A4_C13_Glucose_3SMACA4 repstration + C13 Glucose 30min
SA2592097_A4_C13_Glucose_2SMACA4 repstration + C13 Glucose 30min
SA2592106_A4_C13_Glucose_1SMACA4 repstration + C13 Glucose 30min
SA2592119_A4_C13_Glucose_4SMACA4 repstration + C13 Glucose 30min
SA25921219_A4_C13_Glutamine_4SMACA4 repstration + C13 Glutamine 60min
SA25921316_A4_C13_Glutamine_1SMACA4 repstration + C13 Glutamine 60min
SA25921417_A4_C13_Glutamine_2SMACA4 repstration + C13 Glutamine 60min
SA25921518_A4_C13_Glutamine_3SMACA4 repstration + C13 Glutamine 60min
SA25921610_A4_GlucoseSMACA4 repstration + Glucose 30min
SA25921720_A4_GlutamineSMACA4 repstration + Glutamine 60min
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