Summary of Study ST002587

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001663. The data can be accessed directly via it's Project DOI: 10.21228/M8R72W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002587
Study TitleStable isotope tracer analysis (SITA) using 13C6-glucose and 13C5-glutamine measured by GC/MS in H1703 cells ± SMARCA4/A2 restoration
Study SummarySMARCA4/2-loss drives the metabolic shift preferring glutamine than glucose to sustain the TCA cycle. SITA using 13C6-glucose confirmed that glucose uptake was increased in H1703 cells upon SMARCA4/A2-restoration. Conversely, the 13C5-glutamine SITA in H1703 cells revealed that SMARCA4/A2-restoration decreased glutamine uptake and utilization via glutaminolysis to fuel the TCA cycle as indicated by reduced glutamine metabolites (glutamate, α-KG, metabolites) and TCA cycle intermediates (succinate, fumarate, malate, aspartate, metabolites)
Institute
McGill University
DepartmentBiochemistry
LaboratorySidong Huang Lab
Last NameFu
First NameZheng
AddressMcIntyre Medical Sciences Building, 3655 promenade Sir-William-Osler
Emailzheng.fu2@mail.mcgill.ca
Phone5143985446
Submit Date2023-04-26
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailGC-MS
Release Date2023-05-22
Release Version1
Zheng Fu Zheng Fu
https://dx.doi.org/10.21228/M8R72W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA25921843_Ala_C13_Gln_3Alanine 18h and C13 Glutamine 60min
SA25921941_Ala_C13_Gln_1Alanine 18h and C13 Glutamine 60min
SA25922042_Ala_C13_Gln_2Alanine 18h and C13 Glutamine 60min
SA25922144_Ala_C13_Gln_4Alanine 18h and C13 Glutamine 60min
SA25922245_Ala_GlnAlanine 18h and Glutamine 60min
SA2592232_C13_Glu_2C13 Glucose 30min
SA2592241_C13_Glu_1C13 Glucose 30min
SA2592253_C13_Glu_3C13 Glucose 30min
SA2592264_C13_Glu_4C13 Glucose 30min
SA25922722_C13_Gln_2C13 Glutamine 60min
SA25922824_C13_Gln_4C13 Glutamine 60min
SA25922921_C13_Gln_1C13 Glutamine 60min
SA25923023_C13_Gln_3C13 Glutamine 60min
SA2592315_GluGlucose 30min
SA25923225_GlnGlutamine 60min
SA25923313_A2_C13_Glu_3SMACA2 restoration + C13 Glucose 30min
SA25923412_A2_C13_Glu_2SMACA2 restoration + C13 Glucose 30min
SA25923511_A2_C13_Glu_1SMACA2 restoration + C13 Glucose 30min
SA25923614_A2_C13_Glu_4SMACA2 restoration + C13 Glucose 30min
SA25923731_A2_C13_Gln_1SMACA2 restoration + C13 Glutamine 60min
SA25923834_A2_C13_Gln_4SMACA2 restoration + C13 Glutamine 60min
SA25923933_A2_C13_Gln_3SMACA2 restoration + C13 Glutamine 60min
SA25924032_A2_C13_Gln_2SMACA2 restoration + C13 Glutamine 60min
SA25924115_A2_GluSMACA2 restoration + Glucose 30min
SA25924235_A2_GlnSMACA2 restoration + Glutamine 60min
SA25924316_A4A2_C13_Glu_1SMACA4 and A2 restoration + C13 Glucose 30min
SA25924418_A4A2_C13_Glu_3SMACA4 and A2 restoration + C13 Glucose 30min
SA25924517_A4A2_C13_Glu_2SMACA4 and A2 restoration + C13 Glucose 30min
SA25924619_A4A2_C13_Glu_4SMACA4 and A2 restoration + C13 Glucose 30min
SA25924737_A4A2_C13_Gln_2SMACA4 and A2 restoration + C13 Glutamine 60min
SA25924839_A4A2_C13_Gln_4SMACA4 and A2 restoration + C13 Glutamine 60min
SA25924938_A4A2_C13_Gln_3SMACA4 and A2 restoration + C13 Glutamine 60min
SA25925036_A4A2_C13_Gln_1SMACA4 and A2 restoration + C13 Glutamine 60min
SA25925120_A4A2_GluSMACA4 and A2 restoration + Glucose 30min
SA25925240_A4A2_GlnSMACA4 and A2 restoration + Glutamine 60min
SA2592536_A4_C13_Glu_1SMACA4 restoration + C13 Glucose 30min
SA2592548_A4_C13_Glu_3SMACA4 restoration + C13 Glucose 30min
SA2592557_A4_C13_Glu_2SMACA4 restoration + C13 Glucose 30min
SA2592569_A4_C13_Glu_4SMACA4 restoration + C13 Glucose 30min
SA25925726_A4_C13_Gln_1SMACA4 restoration + C13 Glutamine 60min
SA25925829_A4_C13_Gln_4SMACA4 restoration + C13 Glutamine 60min
SA25925928_A4_C13_Gln_3SMACA4 restoration + C13 Glutamine 60min
SA25926027_A4_C13_Gln_2SMACA4 restoration + C13 Glutamine 60min
SA25926110_A4_GluSMACA4 restoration + Glucose 30min
SA25926230_A4_GlnSMACA4 restoration + Glutamine 60min
Showing results 1 to 45 of 45
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