Summary of Study ST002739
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001704. The data can be accessed directly via it's Project DOI: 10.21228/M8FM85 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002739 |
| Study Title | Metabolic effect of Lamin A/C in oligodendrocyte on brain function |
| Study Type | LC-MS/MS metabolomics of cell type specific Lmna conditional knockout and wildtype mice brains at 26 weeks |
| Study Summary | Oligodendrocytes are specialized cells which insulate and support axons with their myelin membrane, allowing proper brain function. Here, we identify Lamin A/C (LMNA/C) as essential for transcriptional and functional stability of myelinating oligodendrocytes. We show that LMNA/C levels increase with differentiation of progenitors and that loss of Lmna in differentiated oligodendrocytes profoundly alters their chromatin accessibility and transcriptional signature. Lmna deletion in myelinating glia is compatible with normal developmental myelination. However, altered chromatin accessibility is detected in fully differentiated oligodendrocytes together with increased expression of progenitor genes and decreased levels of lipid-related transcription factors and inner mitochondrial membrane transcripts. As mice age, they start to develop myelin-thinning and progressively worsening motor phenotype. To address the metabolic effect of LMNA/C in oligodendrocyte on brain function, we carried out LC-MS/MS metabolomic study of myelinating glia cell specific Lmna conditional knockout and wildtype mice brains at 26 weeks. Each LC-MS/MS experiment was performed with 3 biological replicates and 4 technical replicates per genotype. Overall, our data identify LMNA/C as essential for maintaining the transcriptional and functional stability of myelinating oligodendrocytes. |
| Institute | Advanced Science Research Center - CUNY |
| Department | Neuroscience |
| Laboratory | Casaccia lab, He lab, MALDI and MS core. |
| Last Name | He |
| First Name | Ye |
| Address | 85 St. Nicholas Terrace, New York, New York, 10031, USA |
| yhe1@gc.cuny.edu | |
| Phone | 2124133182 |
| Submit Date | 2023-06-20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-06-22 |
| Release Version | 1 |
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Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | genotype |
|---|---|---|
| SA288788 | KO3_002 | Lmna Knock-Out |
| SA288789 | KO3_003 | Lmna Knock-Out |
| SA288790 | KO1_001 | Lmna Knock-Out |
| SA288791 | KO2_004 | Lmna Knock-Out |
| SA288792 | KO3_004 | Lmna Knock-Out |
| SA288793 | KO3_001 | Lmna Knock-Out |
| SA288794 | KO2_003 | Lmna Knock-Out |
| SA288795 | KO1_002 | Lmna Knock-Out |
| SA288796 | KO1_004 | Lmna Knock-Out |
| SA288797 | KO1_003 | Lmna Knock-Out |
| SA288798 | KO2_001 | Lmna Knock-Out |
| SA288799 | KO2_002 | Lmna Knock-Out |
| SA288800 | WT3_002 | Wild-type |
| SA288801 | WT3_001 | Wild-type |
| SA288802 | WT3_004 | Wild-type |
| SA288803 | WT2_004 | Wild-type |
| SA288804 | WT3_003 | Wild-type |
| SA288805 | WT1_001 | Wild-type |
| SA288806 | WT1_003 | Wild-type |
| SA288807 | WT1_002 | Wild-type |
| SA288808 | WT1_004 | Wild-type |
| SA288809 | WT2_001 | Wild-type |
| SA288810 | WT2_002 | Wild-type |
| SA288811 | WT2_003 | Wild-type |
| Showing results 1 to 24 of 24 |
