Summary of Study ST003060
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001906. The data can be accessed directly via it's Project DOI: 10.21228/M8BQ6H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003060 |
| Study Title | Fluxomics of hormone deprivation in ER+ breast cancer cell lines |
| Study Type | Fluxomics |
| Study Summary | Despite adjuvant treatment with endocrine therapies, estrogen receptor-positive (ER+) breast cancers recur in a significant proportion of patients. Recurrences are attributable to clinically undetectable endocrine-tolerant persister cancer cells that retain tumor-forming potential. We observed that persistence occurred stochastically without genetic predisposition. Genome-wide screening in persisters revealed a survival mechanism involving metabolic reprogramming with reliance upon mitochondrial respiration. Proteomic profiling showed reduced levels of glycolytic proteins in persisters. Metabolic tracing of glucose revealed an energy-depleted state in persisters where oxidative phosphorylation was required to generate ATP. Persisters exhibiting residual proliferation in human breast tumors following neoadjuvant endocrine therapy showed increased mitochondrial content. Pharmacological inhibition of oxidative phosphorylation suppressed the tumor-forming potential of persisters and synergized with the anti-estrogen fulvestrant to induce regression of patient-derived xenografts, supporting therapeutic targeting of mitochondrial metabolism to help eradicate residual disease. |
| Institute | Dartmouth College |
| Department | Molecular and Systems Biology |
| Laboratory | Miller |
| Last Name | Miller |
| First Name | Todd |
| Address | Hanover, NH |
| Todd.W.Miller@Dartmouth.edu | |
| Phone | 603-646-5507 |
| Submit Date | 2024-01-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-02-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | 13C label | Hormone treatment |
|---|---|---|---|
| SA331845 | Blank | Blank | Blank |
| SA331847 | MCF7 HD glucose non-labeled control | glucose | No |
| SA331848 | MCF7 HD glucose_3 | glucose | No |
| SA331849 | MCF7 HD glucose_1 | glucose | No |
| SA331850 | MCF7 HD glucose_2 | glucose | No |
| SA331851 | MCF7 E2 glucose_2 | glucose | Yes |
| SA331852 | MCF7 E2 glucose_1 | glucose | Yes |
| SA331853 | MCF7 E2 glucose_3 | glucose | Yes |
| SA331854 | MCF7 E2 glucose non-labeled control | glucose | Yes |
| SA331855 | MCF7 HD oleic acid non-labeled control | oleic acid | No |
| SA331856 | MCF7 HD oleic acid_1 | oleic acid | No |
| SA331857 | MCF7 HD oleic acid_3 | oleic acid | No |
| SA331858 | MCF7 HD oleic acid_2 | oleic acid | No |
| SA331859 | MCF7 E2 oleic acid_1 | oleic acid | Yes |
| SA331860 | MCF7 E2 oleic acid_2 | oleic acid | Yes |
| SA331861 | MCF7 E2 oleic acid_3 | oleic acid | Yes |
| SA331862 | MCF7 E2 oleic acid non-labeled control | oleic acid | Yes |
| SA331846 | Pool | Pool | Pool |
| Showing results 1 to 18 of 18 |
